RNA-primed allele-specific PCR
文献类型：期刊论文
| 作者 | Zhang LingHui ; Tang Zhuo |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:7页码:961-965 |
| 关键词 | RNA primer Vent(exo-) DNA polymerase TaqM1 DNA polymerase allele-specific PCR single-nucleotide mutation |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | RNA/DNA primer pairs and two polymerases were used to efficiently amplify DNA sequences using the conventional polymerase chain reaction (PCR). The reaction required the use of both DNA polymerase and reverse transcriptase during each thermal cycle and formed a double-stranded DNA in which one terminus was an RNA/DNA hybrid. Because there is a higher sensitivity of the DNA polymerase to the mismatch at the 3'-end in the RNA/DNA hybrid duplex than in the DNA/DNA duplex, the RNA-primed PCR reveals much better specificity in the allele-specific PCR to detect single-nucleotide mutation. |
| 学科主题 | Chemistry, Multidisciplinary |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26677]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhang LingHui,Tang Zhuo. RNA-primed allele-specific PCR[J]. SCIENCE CHINA-CHEMISTRY,2014,57(7):961-965. |
| APA | Zhang LingHui,&Tang Zhuo.(2014).RNA-primed allele-specific PCR.SCIENCE CHINA-CHEMISTRY,57(7),961-965. |
| MLA | Zhang LingHui,et al."RNA-primed allele-specific PCR".SCIENCE CHINA-CHEMISTRY 57.7(2014):961-965. |
入库方式： OAI收割
来源：成都生物研究所
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