Template-directed Chemical Ligation to Obtain 3 '-3 ' and 5 '-5 ' Phosphodiester DNA Linkages
文献类型：期刊论文
| 作者 | Chen, Haodong ; Du, Feng ; Chen, Gangyi ; Streckenbach, Frank ; Yasmeen, Afshan ; Zhao, Yun ; Tang, Zhuo |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 4页码:- |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Up to now, the direct ligation of two DNA fragments with opposite directions to obtain 3'-3' or 5'-5' phosphate ester bonds is still challenging. The only way to obtain DNA oligonucleotides containing a 3'-3' or 5'-5' inversion of polarity sites is based on professional DNA chemical synthesis. Herein, we demonstrate a convenient template-directed chemical ligation that enables 3'-3' and 5'-5' linkages of two DNA oligonucleotides. This method is based on the assembly of two oligonucleotides on a template in opposite directions through forming antiparallel and parallel duplexes simultaneously, followed by coupling with N-Cyanoimidazole under mild condition. Moreover, on the basis of DNA oligonucleotides with 5'-5' linkage obtained through our template-directed chemical ligation, we developed a new cDNA display technique for in vitro selection of functional polypeptides. |
| 学科主题 | Multidisciplinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26689]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Chen, Haodong,Du, Feng,Chen, Gangyi,et al. Template-directed Chemical Ligation to Obtain 3 '-3 ' and 5 '-5 ' Phosphodiester DNA Linkages[J]. SCIENTIFIC REPORTS,2014,4:-. |
| APA | Chen, Haodong.,Du, Feng.,Chen, Gangyi.,Streckenbach, Frank.,Yasmeen, Afshan.,...&Tang, Zhuo.(2014).Template-directed Chemical Ligation to Obtain 3 '-3 ' and 5 '-5 ' Phosphodiester DNA Linkages.SCIENTIFIC REPORTS,4,-. |
| MLA | Chen, Haodong,et al."Template-directed Chemical Ligation to Obtain 3 '-3 ' and 5 '-5 ' Phosphodiester DNA Linkages".SCIENTIFIC REPORTS 4(2014):-. |
入库方式： OAI收割
来源：成都生物研究所
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