Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe
文献类型：期刊论文
| 作者 | Long, Jun ; Wang, Xing-ming ; Xu, Dong-ling ; Ding, Li-sheng |
| 刊名 | JOURNAL OF MOLECULAR RECOGNITION
 |
| 出版日期 | 2014 |
| 卷号 | 27期号:3页码:131-137 |
| 关键词 | safranin T fluorescence spectra DNA interaction acridine orange |
| 产权排序 | 2 |
| 通讯作者 | Wang, XM (reprint author), Southwest Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Under the condition of physiological pH environment (pH=7.40), the interactions of safranin T (ST) with herring sperm DNA were studied by means of spectral methods using acridine orange (AO) as a fluorescence probe. The spectroscopic characteristics of DNA-AO in the case of ST (along with the increase of concentration) were observed in an aqueous medium. The binding constants for ST stranded DNA and competitive bindings of ST interacting with DNA-AO systems were examined by fluorescence spectra, and the binding mechanism of ST with DNA was researched via viscosity measurements. All the testimony manifested that bonding modes between ST and DNA were evidenced to be intercalative binding and electrostatic binding, and the combining constant of ST with DNA was obtained. The binding of ST to DNA was driven by entropy and enthalpy through the calculated thermodynamic parameters (H-r(m)?, S-r(m) and (r)G(m)(?)). Copyright (c) 2014 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26696]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Long, Jun,Wang, Xing-ming,Xu, Dong-ling,et al. Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe[J]. JOURNAL OF MOLECULAR RECOGNITION,2014,27(3):131-137. |
| APA | Long, Jun,Wang, Xing-ming,Xu, Dong-ling,&Ding, Li-sheng.(2014).Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe.JOURNAL OF MOLECULAR RECOGNITION,27(3),131-137. |
| MLA | Long, Jun,et al."Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe".JOURNAL OF MOLECULAR RECOGNITION 27.3(2014):131-137. |
入库方式： OAI收割
来源：成都生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。