In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity
文献类型：期刊论文
| 作者 | Wang, Mingqi ; Zhang, Huafan ; Zhang, Wei ; Zhao, Yongyun ; Yasmeen, Afshan ; Zhou, Li ; Yu, Xiaoqi ; Tang, Zhuo |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 42期号:14页码:9262-9269 |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Single-nucleotide polymorphisms, either inherited or due to spontaneous DNA damage, are associated with numerous diseases. Developing tools for site-specific nucleotide modification may one day provide a way to alter disease polymorphisms. Here, we describe the in vitro selection and characterization of a new deoxyribozyme called F-8, which catalyzes nucleotide excision specifically at thymidine. Cleavage by F-8 generates 3'- and 5'-phosphate ends recognized by DNA modifying enzymes, which repair the targeted deoxyribonucleotide while maintaining the integrity of the rest of the sequence. These results illustrate the potential of DNAzymes as tools for DNA manipulation. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26697]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Wang, Mingqi,Zhang, Huafan,Zhang, Wei,et al. In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity[J]. NUCLEIC ACIDS RESEARCH,2014,42(14):9262-9269. |
| APA | Wang, Mingqi.,Zhang, Huafan.,Zhang, Wei.,Zhao, Yongyun.,Yasmeen, Afshan.,...&Tang, Zhuo.(2014).In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity.NUCLEIC ACIDS RESEARCH,42(14),9262-9269. |
| MLA | Wang, Mingqi,et al."In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity".NUCLEIC ACIDS RESEARCH 42.14(2014):9262-9269. |
入库方式： OAI收割
来源：成都生物研究所
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