Pluronic (R) L64-mediated stable HIF-1 alpha expression in muscle for therapeutic angiogenesis in mouse hindlimb ischemia
文献类型：期刊论文
| 作者 | Song, Hongmei ; Liu, Sijia ; Li, Caixia ; Geng, Yanyan ; Wang, Gang ; Gu, Zhongwei |
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:1页码:3439-3452 |
| 关键词 | gene therapy Pluronic L64 angiogenesis SV40 enhancer HIF-1 alpha |
| 产权排序 | 2 |
| 通讯作者 | Gu, ZW (reprint author), Sichuan Univ, Natl Engn Res Ctr Biomat, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Intramuscular injection of plasmid DNA (pDNA) to express a therapeutic protein is a promising method for the treatment of many diseases. However, the therapeutic applications are usually hindered by gene delivery efficiency and expression level. In this study, critical factors in a pDNA-based gene therapy system, such as gene delivery materials, a therapeutic gene, and its regulatory elements, were optimized to establish an integrated system for the treatment of mouse hindlimb ischemia. The results showed that Pluronic (R) L64 ( L64) was an efficient and safe material for gene delivery into mouse skeletal muscle. It also showed intrinsic ability to promote in vivo angiogenesis in a concentration-dependent manner, which might be through the activation of nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B)-regulated angiogenic factors. The combination of 0.1% L64 with a hybrid gene promoter (pSC) increased the gene expression level, elongated the gene expression duration, and enhanced the number of transfected muscle fibers. In mice ischemic limbs, a gene medicine (pSC-HIF1 alpha(tri)/L64) composed of L64 and pSC-based expression plasmid encoding hypoxia-inducible factor 1-alpha triple mutant (HIF-1 alpha(tri)), improved the expression of stable HIF-1 alpha, and in turn, the expression of multiple angiogenic factors. As a result, the ischemic limbs showed accelerated function recovery, reduced foot necrosis, faster blood reperfusion, and higher capillary density. These results indicated that the pSC-HIF1 alpha(tri)/L64 combination presented a potential and convenient venue for the treatment of peripheral vascular diseases, especially critical limb ischemia. |
| 学科主题 | Nanoscience & Nanotechnology; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26704]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Song, Hongmei,Liu, Sijia,Li, Caixia,et al. Pluronic (R) L64-mediated stable HIF-1 alpha expression in muscle for therapeutic angiogenesis in mouse hindlimb ischemia[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2014,9(1):3439-3452. |
| APA | Song, Hongmei,Liu, Sijia,Li, Caixia,Geng, Yanyan,Wang, Gang,&Gu, Zhongwei.(2014).Pluronic (R) L64-mediated stable HIF-1 alpha expression in muscle for therapeutic angiogenesis in mouse hindlimb ischemia.INTERNATIONAL JOURNAL OF NANOMEDICINE,9(1),3439-3452. |
| MLA | Song, Hongmei,et al."Pluronic (R) L64-mediated stable HIF-1 alpha expression in muscle for therapeutic angiogenesis in mouse hindlimb ischemia".INTERNATIONAL JOURNAL OF NANOMEDICINE 9.1(2014):3439-3452. |
入库方式： OAI收割
来源：成都生物研究所
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