细胞膜结合的雌激素受体的特异性标记
文献类型：期刊论文
| 作者 | 袁韵 ; 林之蔚 ; 郭加加 ; 王飞 ; 袁小红 |
| 刊名 | 应用与环境生物学报
 |
| 出版日期 | 2014 |
| 卷号 | 20期号:2页码:227-232 |
| 关键词 | 雌激素受体 磷酸泛酰巯基乙胺基转移酶 标记 辅酶A |
| 其他题名 | Specififi c labeling of the cell membrane-bound estrogen receptor |
| 产权排序 | 2 |
| 通讯作者 | 袁小红 |
| 合作状况 | 其它 |
| 中文摘要 | 细胞膜结合的雌激素受体(Cell membrane-bound estrogen receptor,cmER)介导了雌激素的非基因组作用,参与了乳腺癌等诸多疾病的发生,但是作用机制研究受限于能够有效区分cmER和细胞质内ER(Estrogen receptor)的标记手段.为了实现cmER的特异性标记,首先在体外分别表达含有A1、S6和ybbR三个不同短肽的谷胱甘肽S转移酶融合蛋白,比较磷酸泛酰巯基乙胺基转移酶(Phosphopantetheinyl transferase,PPTase)-AcpS和Sfp对其的识别选择性.选取识别效率较高和特异性较好的A1短肽,将其构建到雌激素受体ERα的C... |
| 英文摘要 | Cell membrane-bound estrogen receptors (cmERs) mediate the non-genomic effects of estrogen, which is involved in the occurrence of breast cancer and many other estrogen-related diseases. However, the mechanism study is hindered by the lack of a specifi c labeling method which can effi ciently differentiate between cmERs and cytoplasmic ERs. The purpose of this study was to develop the specifi c labeling of the cmER. We expressed and purifi ed glutathione S-transferase fusion proteins which contained three different specifi c peptides A1, S6 and ybbR. Then we compared the labeling specifi city and effi ciency of the proteins by two different phosphopantetheinyl transferase (PPTase): AcpS and Sfp. A1 peptide, which was shown with higher labeling efficiency, was constructed into the C-terminus of the estrogen receptor α (ERα-A1) and then the ERα-A1 expression, its genomic and nongenomic function and labeling of biotin by AcpS were measured in cells. The results showed that coupling of A1 tag did not affect the ER protein expression or its activity. AcpS could specifi cally label biotin to cmERα- A1with the coenzyme A-biotin as a substrate. These results provide a new method for the study of cmER-mediated estrogen nongenomic signal transduction. |
| 学科主题 | 天然产物研究 |
| 收录类别 | CSCD |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5138063 |
| 源URL | [http://210.75.237.14/handle/351003/26712]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | 袁韵,林之蔚,郭加加,等. 细胞膜结合的雌激素受体的特异性标记[J]. 应用与环境生物学报,2014,20(2):227-232. |
| APA | 袁韵,林之蔚,郭加加,王飞,&袁小红.(2014).细胞膜结合的雌激素受体的特异性标记.应用与环境生物学报,20(2),227-232. |
| MLA | 袁韵,et al."细胞膜结合的雌激素受体的特异性标记".应用与环境生物学报 20.2(2014):227-232. |
入库方式： OAI收割
来源：成都生物研究所
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