Production of N-15-labeled alpha-amanitin in Galerina marginata
文献类型：期刊论文
| 作者 | Luo,Hong; DuBois,Brandon; Sgambelluri,R. Michael; Angelos,Evan R.; Li,Xuan; Holmes,Daniel; Walton,Jonathan D. |
| 刊名 | TOXICON
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 103页码:60-64 |
| 关键词 | Amanita Galerina alpha-Amanitin Isotope labeling LC-MS NMR |
| 英文摘要 | alpha-Amanitin is the major causal constituent of deadly Amanita mushrooms that account for the majority of fatal mushroom poisonings worldwide. It is also an important biochemical tool for the study of its target, RNA polymerase II. The commercial supply of this bicyclic peptide comes from Amanita phalloides, the death cap mushroom, which is collected from the wild. Isotopically labeled amanitin could be useful for clinical and forensic applications, but alpha-amanitin has not been chemically synthesized and A. phalloides cannot be cultured on artificial medium. Using Galerina marginata, an unrelated saprotrophic mushroom that grows and produces alpha-amanitin in culture, we describe a method for producing N-15-labeled alpha-amanitin using growth media containing N-15 as sole nitrogen source. A key to success was preparing N-15-enriched yeast extract via a novel method designated "glass bead-assisted maturation." In the presence of the labeled yeast extract and 15N NH4Cl, alpha-amanitin was produced with >97% isotope enrichment. The labeled product was confirmed by HPLC, high-resolution mass spectrometry, and NMR. (C) 2015 Elsevier Ltd. All rights reserved. |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | LIQUID-CHROMATOGRAPHY ; FERMENTATIVE PRODUCTION ; PROLYL OLIGOPEPTIDASE ; STABLE ISOTOPES ; LC-MS/MS ; AMATOXINS ; TOXINS ; BIOSYNTHESIS ; PHALLOTOXINS ; VALIDATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359888300008 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/25107]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 推荐引用方式 GB/T 7714 | Luo,Hong,DuBois,Brandon,Sgambelluri,R. Michael,et al. Production of N-15-labeled alpha-amanitin in Galerina marginata[J]. TOXICON,2015,103:60-64. |
| APA | Luo,Hong.,DuBois,Brandon.,Sgambelluri,R. Michael.,Angelos,Evan R..,Li,Xuan.,...&Walton,Jonathan D..(2015).Production of N-15-labeled alpha-amanitin in Galerina marginata.TOXICON,103,60-64. |
| MLA | Luo,Hong,et al."Production of N-15-labeled alpha-amanitin in Galerina marginata".TOXICON 103(2015):60-64. |
入库方式： OAI收割
来源：昆明植物研究所
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