Isolation and Functional Analysis of SpWOX13 from Stipa purpurea
文献类型：期刊论文
| 作者 | Sun,Xudong; Xiang,Nan; Wang,Chongde; Yang,Shihai; Li,Xiong ; Yang,Yunqiang; Yang,Yongping
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| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 33期号:5页码:1441-1450 |
| 关键词 | SpWOX13 Transcription factor Cell differentiation Stipa purpurea Arabidopsis |
| 英文摘要 | Members of the WUSCHEL-related homeobox (WOX) gene family encode plant-specific transcription factors that play key roles in controlling gene expression at all stages of plant development. In the present study, we isolated and characterized SpWOX13 (GenBank accession no. KJ713172), a member of the WOX gene family, from Stipa purpurea. Sequence and phylogenetic analyses showed that SpWOX13 belongs to an ancient clade of WOX genes. A subcellular localization analysis indicated that SpWOX13 localizes in the nucleus. The transcript levels of SpWOX13 were higher in young leaves and the vegetative apex than in other tissues of S. purpurea. Ectopic expression of SpWOX13 in Arabidopsis resulted in late flowering plants as these showed more rosette leaves. Histological analyses of the transgenic plants showed aberrant arrangements in the tunica cell layers of the shoot apical meristem. Scanning electron microscopy analyses revealed that the leaf pavement cells were larger in SpWOX13-overexpressing transgenic plants than in wild type. Our results suggest that SpWOX13 is involved in delaying and/or inhibiting cell differentiation. |
| 类目[WOS] | Biochemical Research Methods ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | ARABIDOPSIS-THALIANA ; HOMEOBOX GENE ; CELL FATE ; APICAL MERISTEM ; WUS EXPRESSION ; SHOOT MERISTEM ; WUSCHEL ; LEAF ; PROLIFERATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360392800022 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/25112]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun,Xudong,Xiang,Nan,Wang,Chongde,et al. Isolation and Functional Analysis of SpWOX13 from Stipa purpurea[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(5):1441-1450. |
| APA | Sun,Xudong.,Xiang,Nan.,Wang,Chongde.,Yang,Shihai.,Li,Xiong.,...&Yang,Yongping.(2015).Isolation and Functional Analysis of SpWOX13 from Stipa purpurea.PLANT MOLECULAR BIOLOGY REPORTER,33(5),1441-1450. |
| MLA | Sun,Xudong,et al."Isolation and Functional Analysis of SpWOX13 from Stipa purpurea".PLANT MOLECULAR BIOLOGY REPORTER 33.5(2015):1441-1450. |
入库方式： OAI收割
来源：昆明植物研究所
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