Metabolic engineering of Escherichia coli to improve recombinant protein production
文献类型：期刊论文
| 作者 | Liu, Min1,2; Feng, Xinjun1 ; Ding, Yamei3; Zhao, Guang1; Liu, Huizhou1; Xian, Mo1
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| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 99期号:24页码:10367-10377 |
| 关键词 | Recombinant protein production Metabolic engineering Workhorse selection Stress factors Carbon flux regulation Growth retardation |
| 英文摘要 | Escherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as the metabolic burden, the carbon source waste, and the cells' physiological deterioration. This article reviews recent approaches for improving recombinant protein production in metabolic engineering, including workhorse selection, stress factor application, and carbon flux regulation. Selecting a suitable host is the first key point for recombinant protein production. In general, it all depends on characteristics of the strains and the target proteins. It will be triggered cells physiological deterioration when the medium is significantly different from the cell's natural environment. Coexpression of stress factors can help proteins to fold into their native conformation. Carbon flux regulation is a direct approach for redirecting more carbon flux toward the desirable pathways and products. However, some undesirable consequences are usually found in metabolic engineering, such as glucose transport inhibition, cell growth retardation, and useless metabolite accumulation. More efficient regulators and platform cell factories should be explored to meet a variety of production demands. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ACETYL-COA SYNTHETASE ; RNA-BINDING PROTEIN ; CRA GENE KNOCKOUT ; B STRAINS REL606 ; TRANSCRIPTIONAL REGULATION ; PYRUVATE-CARBOXYLASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ACETATE ACCUMULATION ; MOLECULAR CHAPERONES ; GLUCOSE-TRANSPORTER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365712300001 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6797]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Min,Feng, Xinjun,Ding, Yamei,et al. Metabolic engineering of Escherichia coli to improve recombinant protein production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(24):10367-10377. |
| APA | Liu, Min,Feng, Xinjun,Ding, Yamei,Zhao, Guang,Liu, Huizhou,&Xian, Mo.(2015).Metabolic engineering of Escherichia coli to improve recombinant protein production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(24),10367-10377. |
| MLA | Liu, Min,et al."Metabolic engineering of Escherichia coli to improve recombinant protein production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.24(2015):10367-10377. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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