A bladder cancer microenvironment simulation system based on a microfluidic co-culture model
文献类型：期刊论文
| 作者 | Liu, Peng-fei1,2; Cao, Yan-wei1,3; Zhang, Shu-dong4; Zhao, Yang1,2; Liu, Xiao-guang1,2; Shi, Hao-qing1,2; Hu, Ke-yao1,2; Zhu, Guan-qun1,2; Ma, Bo5,6 ; Niu, Hai-tao1,3
|
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2015-11-10 |
| 卷号 | 6期号:35页码:37695-37705 |
| 关键词 | microfluidic device bladder cancer microenvironment three-dimensional culture co-culture drug sensitivity |
| 英文摘要 | A tumor microenvironment may promote tumor metastasis and progression through the dynamic interplay between neoplastic cells and stromal cells. In this work, the most representative and significant stromal cells, fibroblasts, endothelial cells, and macrophages were used as vital component elements and combined with bladder cancer cells to construct a bladder cancer microenvironment simulation system. This is the first report to explore bladder cancer microenvironments based on 4 types of cells co-cultured simultaneously. This simulation system comprises perfusion equipment, matrigel channel units, a medium channel and four indirect contact culture chambers, allowing four types of cells to simultaneously interact through soluble biological factors and metabolites. With this system, bladder cancer cells (T24) with a tendency to form a 'reticular' structure under 3 dimensional culture conditions were observed in real time. The microenvironment characteristics of paracrine interactions and cell motility were successfully simulated in this system. The phenotype change process in stromal cells was successfully reproduced in this system by testing the macrophage effector molecule Arg-1. Arg-1 was highly expressed in the simulated tumor microenvironment group. To develop "precision medicine" in bladder cancer therapy, bladder cancer cells were treated with different clinical 'neo-adjuvant' chemotherapy schemes in this system, and their sensitivity differences were fully reflected. This work provides a preliminary foundation for neo-adjuvant chemotherapy in bladder cancer, a theoretical foundation for tumor microenvironment simulation and promotes individual therapy in bladder cancer patients. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Oncology ; Cell Biology |
| 研究领域[WOS] | Oncology ; Cell Biology |
| 关键词[WOS] | TUMOR MICROENVIRONMENT ; BREAST-CANCER ; ANTICANCER THERAPIES ; VASCULOGENIC MIMICRY ; ANGIOGENIC SWITCH ; DRUG-RESISTANCE ; LUNG-CANCER ; CELLS ; HETEROGENEITY ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366113200055 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6837]  |
| 专题 | 青岛生物能源与过程研究所_单细胞中心 |
| 作者单位 | 1.Qingdao Univ, Affiliated Hosp, Dept Urol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Univ, Coll Med, Qingdao 266071, Shandong, Peoples R China 3.Key Urol Lab Qingdao City, Qingdao, Shandong, Peoples R China 4.Peking Univ, Hosp 3, Dept Urol, Beijing 100871, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China 6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Peng-fei,Cao, Yan-wei,Zhang, Shu-dong,et al. A bladder cancer microenvironment simulation system based on a microfluidic co-culture model[J]. ONCOTARGET,2015,6(35):37695-37705. |
| APA | Liu, Peng-fei.,Cao, Yan-wei.,Zhang, Shu-dong.,Zhao, Yang.,Liu, Xiao-guang.,...&Niu, Hai-tao.(2015).A bladder cancer microenvironment simulation system based on a microfluidic co-culture model.ONCOTARGET,6(35),37695-37705. |
| MLA | Liu, Peng-fei,et al."A bladder cancer microenvironment simulation system based on a microfluidic co-culture model".ONCOTARGET 6.35(2015):37695-37705. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。