Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass
文献类型：期刊论文
| 作者 | Cao, Yujin1 ; Niu, Wei2; Guo, Jiantao2; Xian, Mo1; Liu, Huizhou1
|
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-12-16 |
| 卷号 | 5 |
| 英文摘要 | 1,2,4-Butanetriol BT) is a valuable chemical with extensive applications in many different fields. The traditional chemical routes to synthesize BT suffer from many drawbacks, e. g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed to produce BT from xylose, which is a major component of the lignocellulosic biomass. Through the coexpression of a xylose dehydrogenase (CCxylB) and a xylonolactonase (xylC) from Caulobacter crescentus, native E. coli xylonate dehydratase (yjhG), a 2-keto acid decarboxylase from Pseudomonas putida (mdlC) and native E. coli aldehyde reductase (adhP) in E. coli BL21 star(DE3), the recombinant strain could efficiently convert xylose to BT. Furthermore, the competitive pathway responsible for xylose metabolism in E. coli was blocked by disrupting two genes (xylA and EcxylB) encoding xylose isomerase and xyloluse kinase. Under fed-batch conditions, the engineered strain BL21 Delta xylAB/pE-mdlCxylBC&pA-adhPyjhG produced up to 3.92 g/L of BT from 20 g/L of xylose, corresponding to a molar yield of 27.7%. These results suggest that the engineered E. coli has a promising prospect for the large-scale production of BT. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | D-XYLOSE METABOLISM ; ESCHERICHIA-COLI ; XYLONIC ACID ; DEHYDROGENASE ; CHEMICALS ; PATHWAYS ; SEQUENCE ; FUELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366480100002 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/6842]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA |
| 推荐引用方式 GB/T 7714 | Cao, Yujin,Niu, Wei,Guo, Jiantao,et al. Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Cao, Yujin,Niu, Wei,Guo, Jiantao,Xian, Mo,&Liu, Huizhou.(2015).Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass.SCIENTIFIC REPORTS,5. |
| MLA | Cao, Yujin,et al."Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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