Determination of estrogens in water by HPLC-UV using cloud point extraction
文献类型：会议论文
| 作者 | Wang, Ling; Cai, Ya-Qi; He, Bin; Yuan, Chun-Gang; Shen, Da-Zhong; Shao, Jing; Jiang, Gui-Bin |
| 出版日期 | 2006 |
| 会议名称 | China/Japan/Korea Environmental Analytical Chemistry Symposium |
| 会议日期 | SEP 01-03, 2005 |
| 会议地点 | Chiba, JAPAN |
| 关键词 | estrogens cloud point extraction Triton X-114 non-ionic surfactant HPLC-UV |
| 英文摘要 | A method based on cloud point extraction was developed to determine four kinds of estrogens: estriol (E3), estradiol (E2), estrone (El), and progesterone (P) in water by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as extractant solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, preconcentration factors of 99 for E3. 73 for E2. 152 for E I and 86 for P were obtained for 10 mL water sample. The detection of limitation was 0.23 ng mL(-1) for E3, 0.32 ng mL(-1) for E2, 0.25 ng mL(-1) for El and 5.0 ng mL-1 for P. The proposed method was successfully applied to the determination of trace amount of estrogens in wastewater treatment plant (WWTP) effluent water and exposure water with 10 ng mL-1 E2 for toxicological study in our lab. For the case of WWTP effluent water samples, no estrogen was found. The accuracy of the proposed method was tested by recovery measurements of spiked samples and good recoveries of 81.2-99.5% were obtained. (c) 2006 Published by Elsevier B.V. |
| ISSN号 | 0039-9140 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/24740]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Ling,Cai, Ya-Qi,He, Bin,et al. Determination of estrogens in water by HPLC-UV using cloud point extraction[C]. 见:China/Japan/Korea Environmental Analytical Chemistry Symposium. Chiba, JAPAN. SEP 01-03, 2005. |
入库方式： OAI收割
来源：生态环境研究中心
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