Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography
文献类型：会议论文
| 作者 | Yu, H; Mou, SF |
| 出版日期 | 2006 |
| 会议名称 | 18th International Ion Chromatography Symposium |
| 会议日期 | SEP 18-21, 2005 |
| 会议地点 | Montreal, CANADA |
| 关键词 | amino acids carbohydrates temperature retention anion exchange |
| 英文摘要 | Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography (HPAEC) was investigated. Amino acids retention exhibited both exothermic and endothermic behavior. Significant selectivity variations of some weakly retained amino acids were achieved through changing temperature. However, temperature was almost ineffective at changing the selectivity of strongly retained amino acids. Sugars retention exhibited only exothermic behavior. However, individual carbohydrates showed different relative changes in retention time with increased temperature. There is the general regularity, which is the variation of retention time increases as the sequence of monosaccharide, disaccharide, trisaccharide and tetrasaccharide when the column temperature is increased. Selectivity changes of carbohydrates, including elution order reversal, were also achieved by varying temperature. Temperature was effective at changing the selectivity of between amino acids and carbohydrates. In achieving optimal separation of amino acids and carbohydrates, temperature is a valuable tool. (c) 2005 Elsevier B.V. All rights reserved. |
| ISSN号 | 0021-9673 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/24743]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yu, H,Mou, SF. Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography[C]. 见:18th International Ion Chromatography Symposium. Montreal, CANADA. SEP 18-21, 2005. |
入库方式： OAI收割
来源：生态环境研究中心
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