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Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of A beta(42)-induced locomotor decline in Drosophila
文献类型：期刊论文
| 作者 | Liu, HY; Han, M; Li, QY; Zhang, X; Wang, WA; Huang, FD |
| 刊名 | NEUROSCIENCE BULLETIN
 |
| 出版日期 | 2015 |
| 卷号 | 31期号:5页码:541-549 |
| 关键词 | AGE-RELATED IMPAIRMENT NEURODEGENERATIVE DISEASE MODEL MELANOGASTER |
| 通讯作者 | Huang, FD (reprint author), Chinese Acad Sci, Shanghai Adv Res Inst, Lab Syst Biol, Ctr Stem Cell & NanoMed, Shanghai 201210, Peoples R China.,wangwenan312141030@163.com ; huangfude@yahoo.com |
| 英文摘要 | The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) assay has been established by simultaneously photographing the climbing of multiple groups of flies when they are manually tapped down in test tubes. Here, we automated the assay by using a well-controlled electric motor to drive the tapping, and a homemade program to analyze the climbing height of flies. Using the automated RING (aRING) assay, we found that the climbing ability of a strain of wild-type flies, males in particular, declined rapidly before day 21 after eclosion, but slowly from day 21 to 35. We also found that the expression of arctic mutant A beta(42) accelerated the age-dependent decline in the climbing ability of flies. Moreover, using aRING, we examined the effect of third chromosome deficiencies on the accelerated locomotor decline in A beta(42)-expressing flies, and isolated 7 suppressors and 15 enhancers. |
| WOS标题词 | Neurosciences & Neurology |
| 学科主题 | Neurosciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361625500004 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/3931]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Liu, HY,Han, M,Li, QY,et al. Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of A beta(42)-induced locomotor decline in Drosophila[J]. NEUROSCIENCE BULLETIN,2015,31(5):541-549. |
| APA | Liu, HY,Han, M,Li, QY,Zhang, X,Wang, WA,&Huang, FD.(2015).Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of A beta(42)-induced locomotor decline in Drosophila.NEUROSCIENCE BULLETIN,31(5),541-549. |
| MLA | Liu, HY,et al."Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of A beta(42)-induced locomotor decline in Drosophila".NEUROSCIENCE BULLETIN 31.5(2015):541-549. |
入库方式： OAI收割
来源：上海神经科学研究所
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