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Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails
文献类型：期刊论文
| 作者 | Fu, YB; Huang, CW; Xu, XX; Gu, HF; Ye, YQ; Jiang, CZ; Qiu, ZL; Xie, X |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2015 |
| 卷号 | 25期号:9页码:1013-1024 |
| 关键词 | PLURIPOTENT STEM-CELLS ISCHEMIC-HEART-DISEASE IN-VIVO CARDIAC FIBROBLASTS PROGENITOR CELLS SOMATIC-CELLS ADULT HEART TGF-BETA DIFFERENTIATION REPAIR |
| 通讯作者 | Xie, X (reprint author), Tongji Univ, Shanghai Key Lab Signaling & Dis Res, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai 200092, Peoples R China.,xxie@simm.ac.cn |
| 英文摘要 | The direct conversion, or transdifferentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provides promising approaches for cardiac regeneration. However, genetic manipulations raise safety concerns and are thus not desirable in most clinical applications. The discovery of full chemically induced pluripotent stem cells suggest the possibility of replacing transcription factors with chemical cocktails. Here, we report the generation of automatically beating cardiomyocyte-like cells from mouse fibroblasts using only chemical cocktails. These chemical-induced cardiomyocyte-like cells (CiCMs) express cardiomyocyte-specific markers, exhibit sarcomeric organization, and possess typical cardiac calcium flux and electrophysiological features. Genetic lineage tracing confirms the fibroblast origin of these CiCMs. Further studies show the generation of CiCMs passes through a cardiac progenitor stage instead of a pluripotent stage. Bypassing the use of viral-derived factors, this proof of concept study lays a foundation for in vivo cardiac transdifferentiation with pharmacological agents and possibly safer treatment of heart failure. |
| WOS标题词 | Cell Biology |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360765600006 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/3932]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Fu, YB,Huang, CW,Xu, XX,et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails[J]. CELL RESEARCH,2015,25(9):1013-1024. |
| APA | Fu, YB.,Huang, CW.,Xu, XX.,Gu, HF.,Ye, YQ.,...&Xie, X.(2015).Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails.CELL RESEARCH,25(9),1013-1024. |
| MLA | Fu, YB,et al."Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails".CELL RESEARCH 25.9(2015):1013-1024. |
入库方式： OAI收割
来源：上海神经科学研究所
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