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Neuroprotective Effects of Oligodendrocyte Progenitor Cell Transplantation in Premature Rat Brain following Hypoxic-Ischemic Injury
文献类型：期刊论文
| 作者 | Chen, LX; Ma, SM; Zhang, P; Fan, ZC; Xiong, M; Cheng, GQ; Yang, Y; Qiu, ZL; Zhou, WH; Li, J |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:3页码:e0115997-e0115997 |
| 关键词 | NEURAL STEM-CELLS CENTRAL-NERVOUS-SYSTEM SPINAL-CORD PERIVENTRICULAR LEUKOMALACIA CEREBRAL-ISCHEMIA NEONATAL BRAIN BDNF PROTECTS MODEL REMYELINATION DEMYELINATION |
| 通讯作者 | Zhou, WH (reprint author), Fudan Univ, Childrens Hosp, Dept Neonatol, Shanghai 201102, Peoples R China.,zwhchfu@126.com ; lijina_74@163.com |
| 英文摘要 | Periventricular leukomalacia (PVL) is a common ischemic brain injury in premature infants for which there is no effective treatment. The objective of this study was to determine whether transplanted mouse oligodendrocyte progenitor cells (OPCs) have neuroprotective effects in a rat model of PVL. Hypoxia-ischemia (HI) was induced in 3-day-old rat pups by left carotid artery ligation, followed by exposure to 6% oxygen for 2.5 h. Animals were assigned to OPC transplantation or sham control groups and injected with OPCs or PBS, respectively, and sacrificed up to 6 weeks later for immunohistochemical analysis to investigate the survival and differentiation of transplanted OPCs. Apoptosis was evaluated by double immunolabeling of brain sections for caspase-3 and neuronal nuclei (NeuN), while proliferation was assessed using a combination of anti-Nestin and -bromodeoxyuridine antibodies. The expression of brain-derived neurotrophic factor (BDNF) and Bcl-2 was examined 7 days after OPC transplantation. The Morris water maze was used to test spatial learning and memory. The results showed that transplanted OPCs survived and formed a myelin sheath, and stimulated BDNF and Bcl-2 expression and the proliferation of neural stem cells (NSC), while inhibiting HI-induced neuronal apoptosis relative to control animals. Moreover, deficits in spatial learning and memory resulting from HI were improved by OPC transplantation. These results demonstrate an important neuroprotective role for OPCs that can potentially be exploited in cell-based therapeutic approaches to minimize HI-induced brain injury. |
| WOS标题词 | Science & Technology - Other Topics |
| 学科主题 | Multidisciplinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351425400007 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/3956]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Chen, LX,Ma, SM,Zhang, P,et al. Neuroprotective Effects of Oligodendrocyte Progenitor Cell Transplantation in Premature Rat Brain following Hypoxic-Ischemic Injury[J]. PLOS ONE,2015,10(3):e0115997-e0115997. |
| APA | Chen, LX.,Ma, SM.,Zhang, P.,Fan, ZC.,Xiong, M.,...&Li, J.(2015).Neuroprotective Effects of Oligodendrocyte Progenitor Cell Transplantation in Premature Rat Brain following Hypoxic-Ischemic Injury.PLOS ONE,10(3),e0115997-e0115997. |
| MLA | Chen, LX,et al."Neuroprotective Effects of Oligodendrocyte Progenitor Cell Transplantation in Premature Rat Brain following Hypoxic-Ischemic Injury".PLOS ONE 10.3(2015):e0115997-e0115997. |
入库方式： OAI收割
来源：上海神经科学研究所
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