Central prolactin receptors (PRLRs) regulate hepatic insulin sensitivity in mice via signal transducer and activator of transcription 5 (STAT5) and the vagus nerve
文献类型：期刊论文
| 作者 | Xiao, Fei; Xia, Tingting; Lv, Ziquan; Zhang, Qian; Xiao, Yuzhong; Yu, Junjie; Liu, Hao; Deng, Jiali; Guo, Yajie; Wang, Chunxia |
| 刊名 | DIABETOLOGIA
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:10页码:2136-2144 |
| 关键词 | Central nervous system db/db mice Diabetes Hypothalamus Insulin sensitivity Peripheral tissues Prolactin receptor STAT5 Vagus nerve |
| 通讯作者 | Guo, FF (reprint author), Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci,Key Lab Nutr & Metab, 320 Yueyang Rd, Shanghai 200031, Peoples R China. |
| 英文摘要 | Aims/hypothesis Recent studies have revealed the crucial role of the central nervous system (CNS), especially the hypothalamus, in the regulation of insulin sensitivity in peripheral tissues. The aim of our current study was to investigate the possible involvement of hypothalamic prolactin receptors (PRLRs) in the regulation of hepatic insulin sensitivity. Methods We employed overexpression of PRLRs in mouse hypothalamus via intracerebroventricular injection of adenovirus expressing PRLR and inhibition of PRLRs via adenovirus expressing short-hairpin RNA (shRNA) specific for PRLRs in vivo. Selective hepatic vagotomy was employed to verify the important role of the vagus nerve in mediating signals from the brain to peripheral organs. In addition, a genetic insulin-resistant animal model, the db/db mouse, was used in our study to investigate the role of hypothalamic PRLRs in regulating whole-body insulin sensitivity. Results Overexpression of PRLRs in the hypothalamus improved hepatic insulin sensitivity in mice and inhibition of hypothalamic PRLRs had the opposite effect. In addition, we demonstrated that hypothalamic PRLR-improved insulin sensitivity was significantly attenuated by inhibiting the activity of signal transducer and activator of transcription 5 (STAT5) in the CNS and by selective hepatic vagotomy. Finally, overexpression of PRLRs significantly ameliorated insulin resistance in db/db mice. Conclusions/interpretation Our study identifies a novel central pathway involved in the regulation of hepatic insulin sensitivity, mediated by hypothalamic PRLR/STAT5 signalling and the vagus nerve, thus demonstrating an important role for hypothalamic PRLRs under conditions of insulin resistance. |
| 类目[WOS] | Endocrinology & Metabolism |
| 研究领域[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | ENDOPLASMIC-RETICULUM STRESS ; GLUCOSE-HOMEOSTASIS ; MESSENGER-RNA ; HYPOTHALAMIC NUCLEI ; DIABETES-MELLITUS ; ENERGY-BALANCE ; RAT-BRAIN ; PREGNANCY ; LIVER ; RESISTANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341708900016 |
| 公开日期 | 2016-02-26 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/423]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_代谢的遗传与营养调控研究组 |
| 推荐引用方式 GB/T 7714 | Xiao, Fei,Xia, Tingting,Lv, Ziquan,et al. Central prolactin receptors (PRLRs) regulate hepatic insulin sensitivity in mice via signal transducer and activator of transcription 5 (STAT5) and the vagus nerve[J]. DIABETOLOGIA,2014,57(10):2136-2144. |
| APA | Xiao, Fei.,Xia, Tingting.,Lv, Ziquan.,Zhang, Qian.,Xiao, Yuzhong.,...&Guo, Feifan.(2014).Central prolactin receptors (PRLRs) regulate hepatic insulin sensitivity in mice via signal transducer and activator of transcription 5 (STAT5) and the vagus nerve.DIABETOLOGIA,57(10),2136-2144. |
| MLA | Xiao, Fei,et al."Central prolactin receptors (PRLRs) regulate hepatic insulin sensitivity in mice via signal transducer and activator of transcription 5 (STAT5) and the vagus nerve".DIABETOLOGIA 57.10(2014):2136-2144. |
入库方式： OAI收割
来源：上海营养与健康研究所
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