Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs
文献类型：期刊论文
| 作者 | Wang, CG; Zhang, H; Zeng, DD; Sun, WL; Zhang, HL; Aldalbahi, A; Wang, YS; San, LL; Fan, CH; Zuo, XL |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:38页码:15822—15829 |
| ISSN号 | 2040-3364 |
| 关键词 | RESONANCE ENERGY-TRANSFER MOLECULAR BEACONS GOLD NANOPARTICLES LIVING CELLS NUCLEIC-ACID DNA HYBRIDIZATION OLIGONUCLEOTIDE AMPLIFICATION PROBES |
| 通讯作者 | Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China. |
| 英文摘要 | Simultaneous detection of multiple biomarkers has important prospects in the biomedical field. In this work, we demonstrated a novel strategy for the detection of multiple microRNAs (miRNAs) based on gold nanoparticles (Au NPs) and polyadenine (polyA) mediated nanoscale molecular beacon (MB) probes (denoted p-nanoMBs). Novel fluorescent labeled p-nanoMBs bearing consecutive adenines were designed, of which polyA served as an effective anchoring block binding to the surface of Au NPs, and the appended hairpin block formed an upright conformation that favored the hybridization with targets. Using the co-assembling method and the improved hybridization conformation of the hairpin probes, we achieved high selectivity for specifically distinguishing DNA targets from single-base mismatched DNA targets. We also realized multicolor detection of three different synthetic miRNAs in a wide dynamic range from 0.01 nM to 200 nM with a detection limit of 10 pM. What's more, we even detected miRNAs in a simulated serum environment, which indicated that our method could be used in complex media. Compared with the traditional method, our strategy provides a promising alternative method for the qualitative and quantitative detection of miRNAs. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361834100034 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24913]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, CG,Zhang, H,Zeng, DD,et al. Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs[J]. NANOSCALE,2015,7(38):15822—15829. |
| APA | Wang, CG.,Zhang, H.,Zeng, DD.,Sun, WL.,Zhang, HL.,...&Mi, XQ.(2015).Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs.NANOSCALE,7(38),15822—15829. |
| MLA | Wang, CG,et al."Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs".NANOSCALE 7.38(2015):15822—15829. |
入库方式： OAI收割
来源：上海应用物理研究所
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