Integration of Switchable DNA-Based Hydrogels with Surfaces by the Hybridization Chain Reaction
文献类型：期刊论文
| 作者 | Kahn, JS; Trifonov, A; Cecconello, A; Guo, WW; Fan, CH; Willner, I |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2015 |
| 卷号 | 15期号:11页码:7773—7778 |
| 关键词 | CROSS-LINKED HYDROGEL HORSERADISH-PEROXIDASE IMPEDANCE SPECTROSCOPY CONFORMATIONAL SWITCH RESPONSIVE HYDROGELS BINDING-AFFINITY VISUAL DETECTION THIN-FILMS DNAZYME RELEASE |
| ISSN号 | 1530-6984 |
| 通讯作者 | Willner, I (reprint author), Hebrew Univ Jerusalem, Minerva Ctr Complex Biohybrid Syst, Inst Chem, IL-91904 Jerusalem, Israel. |
| 英文摘要 | A novel method to assemble acrylamide/acrydite DNA copolymer hydrogels on surfaces, specifically gold-coated surfaces, is introduced. The method involves the synthesis of two different copolymer chains consisting of hairpin A, HA, modified acrylamide copolymer and hairpin B, H-B, acrylamide copolymer. In the presence of a nucleic acid promoter monolayer associated with the surface, the hybridization chain reaction between the two hairpin-modified polymer chains is initiated, giving rise to the cross-opening of hairpins HA and HB and the formation of a cross-linked hydrogel on the surface. By the cofunctionalization of the H-A- and Ha-modified polymer chains with G-rich DNA tethers that include the G-quadruplex subunits, hydrogels of switchable stiffness are generated. In the presence of K+-ions, the hydrogel associated with the surface is cooperatively cross-linked by duplex units of H-A and H-B, and K+-ion-stabilized G-quadruplex units, giving rise to a stiff hydrogel. The 18-crown-6-ether-stimulated elimination of the K+-ions dissociates the bridging G-quadruplex units, resulting in a hydrogel of reduced stiffness. The duplex/G-quadruplex cooperatively stabilized hydrogel associated with the surface reveals switchable electrocatalytic properties. The incorporation of hemin into the G-quadruplex units electrocatalyzes the reduction of H2O2. The 18-crown-6-ether stimulated dissociation of the hemin/G-quadruplex bridging units leads to a catalytically inactive hydrogel. |
| 语种 | 英语 |
| WOS记录号 | WOS:000364725400090 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24967]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Kahn, JS,Trifonov, A,Cecconello, A,et al. Integration of Switchable DNA-Based Hydrogels with Surfaces by the Hybridization Chain Reaction[J]. NANO LETTERS,2015,15(11):7773—7778. |
| APA | Kahn, JS,Trifonov, A,Cecconello, A,Guo, WW,Fan, CH,&Willner, I.(2015).Integration of Switchable DNA-Based Hydrogels with Surfaces by the Hybridization Chain Reaction.NANO LETTERS,15(11),7773—7778. |
| MLA | Kahn, JS,et al."Integration of Switchable DNA-Based Hydrogels with Surfaces by the Hybridization Chain Reaction".NANO LETTERS 15.11(2015):7773—7778. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。