Mismatch Extension of DNA Polymerases and High-Accuracy Single Nucleotide Polymorphism Diagnostics by Gold Nanoparticle-Improved Isothermal Amplification
文献类型：期刊论文
| 作者 | Chen, F; Zhao, Y; Fan, CH; Zhao, YX |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 87期号:17页码:8718—8723 |
| 关键词 | ROLLING-CIRCLE AMPLIFICATION CENTER-DOT-T NUCLEIC-ACIDS BASE-PAIR THERMODYNAMICS SEQUENCES NMR QUANTIFICATION REPLICATION PROTEINS |
| ISSN号 | 0003-2700 |
| 通讯作者 | Zhao, YX (reprint author), Xi An Jiao Tong Univ, Educ Minist, Sch Life Sci & Technol, Key Lab Biomed Informat Engn, Xianning West Rd, Xian 710049, Shaanxi, Peoples R China. |
| 英文摘要 | Sequence Mismatches may induce nonspecific extension reaction, causing false results for SNP diagnostics. Herein, we. systematically investigated the impact of various 3'-terminal mismatches on isothermal amplification catalyzed by reptesentative DNA polymerases. Despite their diverse efficiencies depending on types of mismatch and kinds, of DNA: polymerase, all 12 kinds of single 3'-terminal mismatches induced the extension reaction. Generally, only several mismatches (primer-template, C-C, G-A, A-G, and A-A) present an observable inhibitory effect on the amplification reaction, whereas other mismatches trigger amplified signals as high as those of Watson-Crick pairs. The related mechanism was deeply discussed, and a primer-design guideline for specific SNP analysis was summarized. Furthermore, we found that the addition of appropriate gold nanoparticles (AuNPs) can significantly inhibit mismatch extension and enhance the amplification specificity. Also the high-accuracy SNP analysis of human blood genomic DNA has been demonstrated by AuNPs-improved isothermal amplification, the result of which was verified by sequencing (the gold standard method for SNP assay). Collectively, this work provides mechanistic insight into mismatch behavior and achieves accurate SNP diagnostics, holding great potential for the application in molecular diagnostics and personalized medicine. |
| 语种 | 英语 |
| WOS记录号 | WOS:000360773100020 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24994]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chen, F,Zhao, Y,Fan, CH,et al. Mismatch Extension of DNA Polymerases and High-Accuracy Single Nucleotide Polymorphism Diagnostics by Gold Nanoparticle-Improved Isothermal Amplification[J]. ANALYTICAL CHEMISTRY,2015,87(17):8718—8723. |
| APA | Chen, F,Zhao, Y,Fan, CH,&Zhao, YX.(2015).Mismatch Extension of DNA Polymerases and High-Accuracy Single Nucleotide Polymorphism Diagnostics by Gold Nanoparticle-Improved Isothermal Amplification.ANALYTICAL CHEMISTRY,87(17),8718—8723. |
| MLA | Chen, F,et al."Mismatch Extension of DNA Polymerases and High-Accuracy Single Nucleotide Polymorphism Diagnostics by Gold Nanoparticle-Improved Isothermal Amplification".ANALYTICAL CHEMISTRY 87.17(2015):8718—8723. |
入库方式： OAI收割
来源：上海应用物理研究所
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