Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection
文献类型：期刊论文
| 作者 | Wan, Y; Wang, PJ; Su, Y; Wang, LH; Pan, D; Aldalbahi, A; Yang, SL; Zuo, XL |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:46页码:25618—25623 |
| 关键词 | ROLLING CIRCLE AMPLIFICATION SEQUENCE-SPECIFIC DETECTION LABEL-FREE SIGNAL AMPLIFICATION STRAND-DISPLACEMENT GOLD NANOPARTICLES FEMTOMOLAR DNA CHRONOCOULOMETRIC DNA CHAIN-REACTION NUCLEIC-ACIDS |
| ISSN号 | 1944-8244 |
| 通讯作者 | Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China. |
| 英文摘要 | Electrochemical DNA (E-DNA) sensors have been greatly developed and play an important role in early diagnosis of different diseases. To determine the extremely low abundance of DNA biomarkers in clinical samples, scientists are making unremitting efforts toward achieving highly sensitive and selective E-DNA sensors. Here, a novel E-DNA sensor was developed taking advantage of the signal amplification efficiency of nanoprobe-initiated enzymatic polymerization (NIEP). In the NIEP based E-DNA sensor, the capture probe DNA was thiolated at its 3'-terminal to be immobilized onto gold electrode, and the nanoprobe was fabricated by 5'-thiol-terminated signal probe DNA conjugated gold nanoparticles (AuNPs). Both of the probes could simultaneously hybridize with the target DNA to form a sandwich structure followed by the terminal deoxynucleotidyl transferase (TdT)-catalyzed elongation of the free 3'-terminal of DNA on the nanoprobe. During the DNA elongation, biotin labels were incorporated into the NIEP-generated long single-stranded DNA (ssDNA) tentacles, leading to specific binding of avidin modified horseradish peroxidase (Av-HRP). Since there are hundreds of DNA probes on the nanoprobe, one hybridization event would generate hundreds of long ssDNA tentacles, resulting in tens of thousands of HRP catalyzed reduction of hydrogen peroxide and sharply increasing electrochemical signals. By employing nanoprobe and TdT, it is demonstrated that the NIEP amplified E-DNA sensor has a detection limit of 10 fM and excellent differentiation ability for even single-base mismatch. |
| 语种 | 英语 |
| WOS记录号 | WOS:000366005600010 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24999]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wan, Y,Wang, PJ,Su, Y,et al. Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(46):25618—25623. |
| APA | Wan, Y.,Wang, PJ.,Su, Y.,Wang, LH.,Pan, D.,...&Zuo, XL.(2015).Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection.ACS APPLIED MATERIALS & INTERFACES,7(46),25618—25623. |
| MLA | Wan, Y,et al."Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection".ACS APPLIED MATERIALS & INTERFACES 7.46(2015):25618—25623. |
入库方式： OAI收割
来源：上海应用物理研究所
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