Novel DNA Polymer for Amplification Pretargeting
文献类型：期刊论文
| 作者 | Li, X; Huang, QQ; Xiao, J; Liu, GZ; Dou, SP; Rusckowski, M; Shi, HC; Liu, YX; Cheng, DF |
| 刊名 | ACS MEDICINAL CHEMISTRY LETTERS
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:9页码:972—976 |
| 关键词 | ANTIBODY ANTIGEN IMMUNOSCINTIGRAPHY CANCER RADIOIMMUNOTHERAPY STREPTAVIDIN MORPHOLINO STRATEGY B72.3 MICE |
| ISSN号 | 1948-5875 |
| 通讯作者 | Liu, YX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China. |
| 英文摘要 | In this Letter, different from conventional pretargeting, an additional novel DNA polymer with multiple copies of a target was first designed to be administrated between the antitumor antibody, and the labeled effector served as an amplification pretargeting strategy. Two phosphorothioate DNA strands, a bridging and a target strand, were hybridized to form a polymer. Polymer size, as a function of molar ratios, was then monitored by size exclusion HPLC and electrophoretic mobility shift assay. Moreover, binding efficiency of polymers with the radiolabeled effector and polymer size after hybridization were measured by HPLC as well. As the polymer was expected to produce more binding sites that would be targeted by effectors, amplification pretargeting can greatly improve accumulation of effectors in tumor. This novel proof-of-concept was then well demonstrated by the in vitro test of signal amplification in antibody-binding protein L coated plate and LS174T cells. Compared to conventional pretargeting, significantly increasing radioactive signal was observed in this designed amplification pretargeting, which would serve as a useful paradigm of the potential of oligomer polymers to improve pretargeting and other related approaches. |
| 语种 | 英语 |
| WOS记录号 | WOS:000361259800006 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25005]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, X,Huang, QQ,Xiao, J,et al. Novel DNA Polymer for Amplification Pretargeting[J]. ACS MEDICINAL CHEMISTRY LETTERS,2015,6(9):972—976. |
| APA | Li, X.,Huang, QQ.,Xiao, J.,Liu, GZ.,Dou, SP.,...&Cheng, DF.(2015).Novel DNA Polymer for Amplification Pretargeting.ACS MEDICINAL CHEMISTRY LETTERS,6(9),972—976. |
| MLA | Li, X,et al."Novel DNA Polymer for Amplification Pretargeting".ACS MEDICINAL CHEMISTRY LETTERS 6.9(2015):972—976. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。