Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes
文献类型：期刊论文
| 作者 | Zhu, D; Pei, H; Chao, J; Su, S; Aldalbahi, A; Rahaman, M; Wang, LH; Wang, LH; Huang, W; Fan, CH |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:44页码:18671—18676 |
| 关键词 | NUCLEIC-ACIDS GENE-REGULATION LIVING CELLS SCAFFOLD PROTEINS NANO-FLARES URANYL-ION DELIVERY OLIGONUCLEOTIDES EFFICIENCY MECHANISM |
| ISSN号 | 2040-3364 |
| 通讯作者 | Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. |
| 英文摘要 | Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal core, which is decorated with a functional shell of DNAzymes. Conventional thiolated DNAzyme-based assembly leads to disordered structures with suppressed activity. In contrast, by using an anchoring block of polyA tails, we find that the activity of SNAzymes can be programmably regulated. By using a polyA30 tail, SNAzymes demonstrated remarkably enhanced binding affinity compared to the thiolated DNAzyme-based assembly (similar to 75-fold) or individual DNAzymes in the solution phase (similar to 10-fold). More significantly, this increased affinity is directly translated to the sensitivity improvement in the SNAzyme-based lead sensor. Hence, this design of SNAzymes may provide new opportunities for developing biosensors and bioimaging probes for theranostic applications. |
| 语种 | 英语 |
| WOS记录号 | WOS:000364824000036 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25016]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhu, D,Pei, H,Chao, J,et al. Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes[J]. NANOSCALE,2015,7(44):18671—18676. |
| APA | Zhu, D.,Pei, H.,Chao, J.,Su, S.,Aldalbahi, A.,...&Zuo, XL.(2015).Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes.NANOSCALE,7(44),18671—18676. |
| MLA | Zhu, D,et al."Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes".NANOSCALE 7.44(2015):18671—18676. |
入库方式： OAI收割
来源：上海应用物理研究所
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