Purification of rectangle DNA origami by rate-zonal centrifugation
文献类型：期刊论文
| 作者 | Chao, J; Xing, S; Feng, BY; Wang, JB; Dai, JB; Wang, LH; Li, Q |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2015 |
| 卷号 | 26期号:5页码:— |
| 关键词 | FOLDING DNA NANOSCALE SHAPES NANOARCHITECTURES NANOSTRUCTURES SEPARATION |
| ISSN号 | 1001-8042 |
| 通讯作者 | Li, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. |
| 英文摘要 | DNA origami technique, a breakthrough in DNA nanotechnology, has been widely used to prepare complex DNA nanostructures with nanoscale addressability. However, the purity and yield are generally the bottleneck to application of DNA nanostructures, and current methods for purifying DNA origami nanostructures in large quantities are time-consuming and laborious. This study aims to develop a scalable, cost-effective and contamination-free method of purifying DNA origami nanostructures. We employ an effective and convenient purification approach to purify planar rectangle DNA origami structures through rate-zonal centrifugation. By subjecting DNA origami samples to high centrifugal force in a density gradient media of glycerol, well-folded nanostructures and by-products are separated successfully, which are confirmed by agarose gel electrophoresis and atomic force microscopy (AFM). This method will aid the production of pure rectangle DNA origami nanostructures in large quantity. |
| 语种 | 英语 |
| WOS记录号 | WOS:000365459200018 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25023]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chao, J,Xing, S,Feng, BY,et al. Purification of rectangle DNA origami by rate-zonal centrifugation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26(5):—. |
| APA | Chao, J.,Xing, S.,Feng, BY.,Wang, JB.,Dai, JB.,...&Li, Q.(2015).Purification of rectangle DNA origami by rate-zonal centrifugation.NUCLEAR SCIENCE AND TECHNIQUES,26(5),—. |
| MLA | Chao, J,et al."Purification of rectangle DNA origami by rate-zonal centrifugation".NUCLEAR SCIENCE AND TECHNIQUES 26.5(2015):—. |
入库方式： OAI收割
来源：上海应用物理研究所
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