Visualizing dopamine released from living cells using a nanoplasmonic probe
文献类型：期刊论文
| 作者 | Qin, WW; Wang, SP; Li, J; Peng, TH; Xu, Y; Wang, K; Shi, JY; Fan, CH; Li, D |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:37页码:15070—15074 |
| ISSN号 | 2040-3364 |
| 关键词 | SINGLE VESICLE RELEASE AU NANOPARTICLES NEUROTRANSMITTER RELEASE GOLD NANOPARTICLES SYNAPTIC VESICLE PC12 CELLS METAL NANOPARTICLES GROWTH MICROELECTRODES NANOSENSORS |
| 通讯作者 | Li, D (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai 201800, Peoples R China. |
| 英文摘要 | We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca2+ influx, and the influx of Ca2+ is through ATP-activated channels instead of the voltage-gated Ca2+ channel (VGC). |
| 语种 | 英语 |
| WOS记录号 | WOS:000361675300007 |
| 公开日期 | 2016-03-08 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25096]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Qin, WW,Wang, SP,Li, J,et al. Visualizing dopamine released from living cells using a nanoplasmonic probe[J]. NANOSCALE,2015,7(37):15070—15074. |
| APA | Qin, WW.,Wang, SP.,Li, J.,Peng, TH.,Xu, Y.,...&Li, D.(2015).Visualizing dopamine released from living cells using a nanoplasmonic probe.NANOSCALE,7(37),15070—15074. |
| MLA | Qin, WW,et al."Visualizing dopamine released from living cells using a nanoplasmonic probe".NANOSCALE 7.37(2015):15070—15074. |
入库方式： OAI收割
来源：上海应用物理研究所
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