Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides
文献类型：期刊论文
| 作者 | Zhao, Qiang; Lv, Qin; Wang, Hailin |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2015-08-15 |
| 卷号 | 70期号:1页码:188-193 |
| 关键词 | Aptamer Fluorescence anisotropy Fluorescence polarization Adenosine triphosphate Fluorescence sensor Labeling site |
| 英文摘要 | We previously reported a fluorescence anisotropy (FA) approach for small molecules using tetramethylrhodamine (TMR) labeled aptamer. It relies on target-binding induced change of intramolecular interaction between TMR and guanine (G) base. TMR-labeling sites are crucial for this approach. Only terminal ends and thymine (T) bases could be tested for TMR labeling in our previous work, possibly causing limitation in analysis of different targets with this FA strategy. Here, taking the analysis of adenosine triphosphate (ATP) as an example, we demonstrated a success of conjugating TMR on other bases of aptamer adenine (A) or cytosine (C) bases and an achievement of full mapping various labeling sites of aptamers. We successfully constructed aptamer fluorescence anisotropy (FA) sensors for adenosine triphosphate (ATP). We conjugated single TMR on adenine (A), cytosine (C), or thymine (T) bases or terminals of a 25-mer aptamer against ATP and tested FA responses of 14 TMR-labeled aptamer to ATP. The aptamers having TMR labeled on the 16th base C or 23rd base A were screened out and exhibited significant FA-decreasing or FA-increasing responses upon ATP, respectively. These two favorable TMR-labeled aptamers enabled direct FA sensing ATP with a detection limit of 1 mu m and the analysis of ATP in diluted serum. The comprehensive screening various TMR labeling sites of aptamers facilitates the successful construction of FA sensors using TMR-labeled aptamers. It will expand application of TMR-G interaction based aptamer FA strategy to a variety of targets. (C) 2015 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS记录号 | WOS:000356554400026 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32516]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao, Qiang,Lv, Qin,Wang, Hailin. Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides[J]. BIOSENSORS & BIOELECTRONICS,2015,70(1):188-193. |
| APA | Zhao, Qiang,Lv, Qin,&Wang, Hailin.(2015).Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides.BIOSENSORS & BIOELECTRONICS,70(1),188-193. |
| MLA | Zhao, Qiang,et al."Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides".BIOSENSORS & BIOELECTRONICS 70.1(2015):188-193. |
入库方式： OAI收割
来源：生态环境研究中心
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