Exploring the diameter and surface dependent conformational changes in carbon nanotube-protein corona and the related cytotoxicity
文献类型：期刊论文
| 作者 | Zhao, Xingchen; Lu, Dawei; Hao, Fang ; Liu, Rutao
|
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2015-07-15 |
| 卷号 | 292页码:98-107 |
| 关键词 | Carbon nanotubes Diameter Serum albumin Nanotoxicology |
| 英文摘要 | In this work, we investigated and compared carbon nanotubes (CNTs) of different diameters regarding their interaction with bovine serum albumin (BSA) and their ability to alter protein structure. BSA was exposed to CNT solutions, and the effects were assessed by utilizing fluorescence spectroscopy, UV-vis absorption spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), bichinchoninic acid (BCA) and zeta-potential measurement assays. We demonstrate that CNT diameter and surface area play key roles in influencing the stability of adsorbed proteins. Results showed that the secondary and tertiary structural stability of BSA decreased upon adsorption onto CNTs, with greater decrease on smaller-diametered nanotubes. Besides, more protein was loaded onto CNTs with small diameter, reducing the cytotoxicity. This study, therefore, provides fundamental information for the influence of CNT diameter and surface on protein behavior, which may be helpful to understand toxic effects of CNTs and prove beneficial for developing novel biomedical devices and safe use of nanomaterials. (C) 2015 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Engineering, Environmental ; Engineering, Civil ; Environmental Sciences |
| WOS记录号 | WOS:000353740500012 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32574]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao, Xingchen,Lu, Dawei,Hao, Fang,et al. Exploring the diameter and surface dependent conformational changes in carbon nanotube-protein corona and the related cytotoxicity[J]. JOURNAL OF HAZARDOUS MATERIALS,2015,292:98-107. |
| APA | Zhao, Xingchen,Lu, Dawei,Hao, Fang,&Liu, Rutao.(2015).Exploring the diameter and surface dependent conformational changes in carbon nanotube-protein corona and the related cytotoxicity.JOURNAL OF HAZARDOUS MATERIALS,292,98-107. |
| MLA | Zhao, Xingchen,et al."Exploring the diameter and surface dependent conformational changes in carbon nanotube-protein corona and the related cytotoxicity".JOURNAL OF HAZARDOUS MATERIALS 292(2015):98-107. |
入库方式： OAI收割
来源：生态环境研究中心
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