G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic
文献类型：期刊论文
| 作者 | Liang, Gang; Liu, Xinhui |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2015-10 |
| 卷号 | 182期号:13-14页码:2233-2240 |
| 关键词 | 2-Hydroxyfluorene Fluorene metabolite G-quadruplex Biosensor Electrochemical impedance spectroscopy Charge transfer resistance |
| 英文摘要 | We describe a sensitive and selective biosensor for the environmental metabolite 2-hydroxyfluorene (2-HOFlu). It is based on electrochemical impedance spectroscopy and was obtained by assembling a thiolated single-stranded DNA on a gold electrode via S-Au covalent bonding. It is then transformed to a K+-stabilized G-quadruplex-hemin complex which exhibits peroxidase-like activity to catalyze the oxidation of 2-HOFlu by H2O2. This results in the formation of insoluble products that are precipitated on the gold electrode. As a result, the charge transfer resistance (R (CT)) between the solution and the electrode surface is strongly increased within 10 min as demonstrated by using the ferro/ferricyanide system as a redox probe. The difference in the charge transfer resistances (Delta R (CT)) before and after incubation of the DNA film with 2-HOFlu and H2O2 serves as the signal for the quantitation of 2-HOFlu with a 1.2. nM detection limit in water of pH 7.4. The assay is highly selective over other selected fluorene derivatives. It was exploited to determine 2-HOFlu in spiked lake water samples where it displayed a detection limit of 3.6 nM. Conceivably, this method has a wide scope in that it may be applied to other analytes for which respective G-quadruplexes are available. |
| 研究领域[WOS] | Chemistry, Analytical |
| WOS记录号 | WOS:000361394900017 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32583]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liang, Gang,Liu, Xinhui. G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic[J]. MICROCHIMICA ACTA,2015,182(13-14):2233-2240. |
| APA | Liang, Gang,&Liu, Xinhui.(2015).G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic.MICROCHIMICA ACTA,182(13-14),2233-2240. |
| MLA | Liang, Gang,et al."G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic".MICROCHIMICA ACTA 182.13-14(2015):2233-2240. |
入库方式： OAI收割
来源：生态环境研究中心
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