Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica
文献类型：期刊论文
| 作者 | Lu, Hua; Lu, Yang; Ren, Jinwei; Wang, Zhongfu; Wang, Qian; Luo, Yuanming; Han, Jing; Xiang, Hua; Du, Yuguo; Jin, Cheng |
| 刊名 | GLYCOBIOLOGY
 |
| 出版日期 | 2015-11 |
| 卷号 | 25期号:11页码:1150-1162 |
| 关键词 | Haloarcula hispanica oligosaccharide S-layer glycoprotein structure |
| 英文摘要 | Haloarcula hispanica is one of members of the Halobacteriaceae, which displays particularly low restriction activity and is therefore important as one of the most tractable haloarchaea for archaeal genetic research. Although the Har. hispanica S-layer protein has been reported glycosylated, the S-layer glycoprotein and its glycosylation have not been investigated yet. In this study, the S-layer proteins of Har. hispanica were extracted and characterized. The S-layer was found containing two different glycoproteins which shared highly similar amino acid sequences. The genes coding for these two S-layer glycoproteins were found next to each other in the genome. Moreover, the N- and O-linked glycans were released from these two S-layer glycoproteins for structural determination. Based on the mass spectrometry and nuclear magnetic resonance, the N-glycan was determined as a branched trisaccharide containing a 225 Da residue corresponded to a 2-amino-6-sulfo-2, 6-dideoxy-quinovose, which was the first time that a naturally occurring form of sulfoquinovosamine was identified. Besides, the O-glycan was characterized as a Glc alpha-1,4-Gal disaccharide by mass spectrometry combined with monosaccharide composition analysis and glycosidase treatment. The determination of the N- and O-glycan structure will be helpful for studying the diverse protein glycosylation pathways in archaea utilizing H. hispanica as a new model. |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000362991500004 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32598]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Lu, Hua,Lu, Yang,Ren, Jinwei,et al. Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica[J]. GLYCOBIOLOGY,2015,25(11):1150-1162. |
| APA | Lu, Hua.,Lu, Yang.,Ren, Jinwei.,Wang, Zhongfu.,Wang, Qian.,...&Jin, Cheng.(2015).Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica.GLYCOBIOLOGY,25(11),1150-1162. |
| MLA | Lu, Hua,et al."Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica".GLYCOBIOLOGY 25.11(2015):1150-1162. |
入库方式： OAI收割
来源：生态环境研究中心
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