Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase
文献类型：期刊论文
| 作者 | Yu, Zehua; Liu, Hongwei; Hu, Xinxin; Song, Wei; Liu, Rutao |
| 刊名 | JOURNAL OF LUMINESCENCE
 |
| 出版日期 | 2015-03 |
| 卷号 | 159页码:312-316 |
| 关键词 | Nanotoxicology Superparamagnetic nanometer iron oxide Catalase Molecular conformation Function |
| 英文摘要 | Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for various applications in targeted drug delivery and magnetic resonance imaging. Given their clinical relevance, there is a need to understand these particles' potential cytotoxic effects and possible mechanisms of cytotoxicity. Using a variety of spectroscopic techniques, we investigated the interaction of SPIONs with catalase (CAT) in an aqueous environment. Catalase is an important enzyme that protects cells and tissues from oxidative damage by reactive oxygen species (ROS). Therefore, in this work, CAT served as a model protein for examining the physiological effects of SPIONs due to is function in eliminating H2O2. Synchronous fluorescence spectroscopy results showed that SPIONs have little effect on tryptophan residues in CAT. Data from circular dichroism (CD) and UV-vis spectroscopies showed that CAT a-helical content decreased from 32.4% to 29.1% in the presence of SPIONs. Moreover, a ca. 10% decrease in CAT activity was observed in the presence of SPIONs at a 20:1 particle:protein ratio. These results show that SPIONs can interact with proteins to alter both their structure and function. Further studies with CAT or other toxicologically relevant enzymes may be used for elucidating the mechanisms of SPION cytotoxicity. (C) 2014 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Optics |
| WOS记录号 | WOS:000348953800043 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32607]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yu, Zehua,Liu, Hongwei,Hu, Xinxin,et al. Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase[J]. JOURNAL OF LUMINESCENCE,2015,159:312-316. |
| APA | Yu, Zehua,Liu, Hongwei,Hu, Xinxin,Song, Wei,&Liu, Rutao.(2015).Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase.JOURNAL OF LUMINESCENCE,159,312-316. |
| MLA | Yu, Zehua,et al."Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase".JOURNAL OF LUMINESCENCE 159(2015):312-316. |
入库方式： OAI收割
来源：生态环境研究中心
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