Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Pan, Susu; Tian, Yong; Li, Ming; Zhao, Jiuyan; Zhu, Lanlan; Zhang, Wei; Gu, Haiwei; Wang, Haidong; Shi, Jianbo; Fang, Xiang |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-03-04 |
| 卷号 | 5页码:- |
| 英文摘要 | Exhaled nitric oxide (eNO) is a useful biomarker of various physiological conditions, including asthma and other pulmonary diseases. Herein a fast and sensitive analytical method has been developed for the quantitative detection of eNO based on extractive electrospray ionization mass spectrometry (EESI-MS). Exhaled NO molecules selectively reacted with 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) reagent, and eNO concentration was derived based on the EESI-MS response of 1-oxyl-2-phenyl-4, 4, 5, 5-tetramethylimidazoline (PTI) product. The method allowed quantification of eNO below ppb level (similar to 0.02 ppbv) with a relative standard deviation (RSD) of 11.6%. In addition, eNO levels of 20 volunteers were monitored by EESI-MS over the time period of 10 hrs. Long-term eNO response to smoking a cigarette was recorded, and the observed time-dependent profile was discussed. This work extends the application of EESI-MS to small molecules (<30 Da) with low proton affinity and collision-induced dissociation efficiency, which are usually poorly visible by conventional ion trap mass spectrometers. Long-term quantitative profiling of eNO by EESI-MS opens new possibilities for the research of human metabolism and clinical diagnosis. |
| 研究领域[WOS] | Multidisciplinary Sciences |
| WOS记录号 | WOS:000350375000005 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32652]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Pan, Susu,Tian, Yong,Li, Ming,et al. Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry[J]. SCIENTIFIC REPORTS,2015,5:-. |
| APA | Pan, Susu.,Tian, Yong.,Li, Ming.,Zhao, Jiuyan.,Zhu, Lanlan.,...&Chen, Huanwen.(2015).Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry.SCIENTIFIC REPORTS,5,-. |
| MLA | Pan, Susu,et al."Quantitative detection of nitric oxide in exhaled human breath by extractive electrospray ionization mass spectrometry".SCIENTIFIC REPORTS 5(2015):-. |
入库方式： OAI收割
来源：生态环境研究中心
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