Real Time Online Correction of Mass Shifts and Intensity Fluctuations in Extractive Electrospray Ionization Mass Spectrometry
文献类型：期刊论文
| 作者 | Tian, Yong; Yu, Miao; Chen, Jian; Liu, Chunxiao; Shi, Jianbo; Chen, Huanwen; Jiang, Guibin |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-12-15 |
| 卷号 | 87期号:24页码:11962-11966 |
| 英文摘要 | Real time online calibration of mass shift and intensity fluctuation to improve the accuracy of measurements for identification and quantitation in trace mass spectrometric analysis was demonstrated using extractive electrospray ionization mass spectrometry (EESI-MS). The signals of authentic compounds (e.g., lysine (Lys), proline (Pro), and histidine (His)) spiked into the extractive solution for the EESI process were used as the references to calibrate the signal of analytes (e.g., methionine (Met)) in the untreated sample solution. The intensity of the analyte signal was recorded simultaneously with the reference signals. The analyte signals at a given time point were calibrated on the basis of these correlation factors and real time signal response of the reference. The calibrated signal of Met at 10 mu g L-1 was improved with a better signal-to-noise ratio (S/N from 2.3 to 4.3), better linearity (R-2 from 0.9758 to 0.9980), and reduced relative standard deviation (RSD from 9.8% to 6.0%). The shift of mass-to-charge ratio of Met signal between the detected and theoretical values was decreased from 247 +/- 133 to -7 +/- 167 ppm for 50 min of detection using a linear ion trap mass analyzer and was reduced from -0.27 +/- 0.60 to -0.12 +/- 0.23 ppm for 50 min of detection using an Orbitrap mass analyzer (P = 95%). This method has been validated using a certified standard amino acids solution (GBW(E)100062) and applied for quantitative detection of amino acids in chicken feed, urine, nutritional drink, and facial mask samples, showing that the method is useful to improve the accuracy of mass spectrometric analysis. |
| 研究领域[WOS] | Chemistry, Analytical |
| WOS记录号 | WOS:000366871500005 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32655]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Tian, Yong,Yu, Miao,Chen, Jian,et al. Real Time Online Correction of Mass Shifts and Intensity Fluctuations in Extractive Electrospray Ionization Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2015,87(24):11962-11966. |
| APA | Tian, Yong.,Yu, Miao.,Chen, Jian.,Liu, Chunxiao.,Shi, Jianbo.,...&Jiang, Guibin.(2015).Real Time Online Correction of Mass Shifts and Intensity Fluctuations in Extractive Electrospray Ionization Mass Spectrometry.ANALYTICAL CHEMISTRY,87(24),11962-11966. |
| MLA | Tian, Yong,et al."Real Time Online Correction of Mass Shifts and Intensity Fluctuations in Extractive Electrospray Ionization Mass Spectrometry".ANALYTICAL CHEMISTRY 87.24(2015):11962-11966. |
入库方式： OAI收割
来源：生态环境研究中心
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