Silver nanoparticle exposure induces rat motor dysfunction through decrease in expression of calcium channel protein in cerebellum
文献类型：期刊论文
| 作者 | Yin, Nuoya; Zhang, Yang; Yun, Zhaojun; Liu, Qian; Qu, Guangbo; Zhou, Qunfang; Hu, Ligang; Jiang, Guibin |
| 刊名 | TOXICOLOGY LETTERS
 |
| 出版日期 | 2015-09-02 |
| 卷号 | 237期号:2页码:112-120 |
| 关键词 | Silver nanoparticles Neurotoxicity Motor coordination dysfunction Locomotor activity impairment Calcium channel protein |
| 英文摘要 | Silver nanoparticles (AgNPs) are currently used widely, however, their impact on central nervous system still remains ambiguous and needs to be elucidated. This study is performed to investigate the neurotoxicity of AgNPs and illustrate the potential molecular mechanism. Neonatal Sprague-Dawley (SD) rats are exposed to AgNPs by intranasal instillation for 14 weeks. It is demonstrated that AgNPs exposure causes cerebellar ataxia like symptom in rats, evidenced by dysfunction of motor coordination and impairment of locomotor activity. Observation of cerebellum section reveals that AgNPs can provoke destruction of cerebellum granular layer with concomitant activation of glial cells. AgNPs treatment decreases calcium channel protein (CACNA1A) levels in cerebellum without changing potassium channel protein (KCNA1) levels. The levels of silver in rat cerebellum tissue are correlated with exposure doses. In vitro experiments reveal that AgNPs treatment significantly reduces the protein and mRNA levels of CACNA1A in primary cultured cerebellum granule cells (CGCs). These findings suggest that AgNPs-induced rat motor dysfunction is associated with CACNA1A expression decrease, which reveals the underlying molecular mechanism for the neurotoxicity of AgNPs. Possible counteractions may accordingly be suggested to attenuate the unexpected harmful effects in biological applications of AgNPs. (C) 2015 Elsevier Ireland Ltd. All rights reserved. |
| 研究领域[WOS] | Toxicology |
| WOS记录号 | WOS:000357391500006 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32660]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yin, Nuoya,Zhang, Yang,Yun, Zhaojun,et al. Silver nanoparticle exposure induces rat motor dysfunction through decrease in expression of calcium channel protein in cerebellum[J]. TOXICOLOGY LETTERS,2015,237(2):112-120. |
| APA | Yin, Nuoya.,Zhang, Yang.,Yun, Zhaojun.,Liu, Qian.,Qu, Guangbo.,...&Jiang, Guibin.(2015).Silver nanoparticle exposure induces rat motor dysfunction through decrease in expression of calcium channel protein in cerebellum.TOXICOLOGY LETTERS,237(2),112-120. |
| MLA | Yin, Nuoya,et al."Silver nanoparticle exposure induces rat motor dysfunction through decrease in expression of calcium channel protein in cerebellum".TOXICOLOGY LETTERS 237.2(2015):112-120. |
入库方式： OAI收割
来源：生态环境研究中心
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