Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro
文献类型：期刊论文
| 作者 | Yang, Rong; Li, Na; Rao, Kaifeng; Ma, Mei; Wang, Zijian |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2015-01 |
| 卷号 | 111页码:228-235 |
| 关键词 | Mixture toxicity Antiestrogen Estrogen Prediction model Two-hybrid yeast bioassay |
| 英文摘要 | Estrogen receptor (ER) antagonistic chemicals in aquatic environments are believed to influence the binding of both endogenous and exogenous estrogens to ERs in aquatic organisms. Although the combined effects of estrogenic compounds have attracted much scientific concern, little work has been done on the influence of such antiestrogens on the biological effects of estrogens. This study focused on how the presence of different amounts of antagonists affects the results of ER agonist activity tests. To achieve this, three questions were stated and answered in sequence. A two-hybrid recombinant yeast assay mediated by ER was adopted, providing a single mode of action and single target of action for this study. Mixtures created by an ER agonist and three antagonists following the fixed-ratio principle were assessed. The concentration of 17 beta-estradiol causing maximum induction was set as the fixed dose of estrogen in the antagonist activity test (question 1). When the two classes of chemicals coexisted, antiestrogens, which as a whole behaved according to the concentration addition model (question 2), decreased the response of estrogen and compressed the concentration-response curves along the y-axis in the agonist activity test (question 3). This may cause the estradiol equivalent to be underestimated and potentially mask the action of estrogenic effects in toxicity evaluation of environmental samples. (C) 2014 Elsevier Inc. All rights reserved. |
| 研究领域[WOS] | Environmental Sciences ; Toxicology |
| WOS记录号 | WOS:000347267800031 |
| 公开日期 | 2016-03-15 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32743]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Rong,Li, Na,Rao, Kaifeng,et al. Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2015,111:228-235. |
| APA | Yang, Rong,Li, Na,Rao, Kaifeng,Ma, Mei,&Wang, Zijian.(2015).Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,111,228-235. |
| MLA | Yang, Rong,et al."Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 111(2015):228-235. |
入库方式： OAI收割
来源：生态环境研究中心
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