Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces
文献类型：期刊论文
| 作者 | Almeida I; Marques JT; Liu W; Niu Y ; de Almeida RFM; Jin G(靳刚); Viana AS
|
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 136页码:997-1003 |
| 通讯作者邮箱 | anaviana@fc.ul.pt |
| 关键词 | Planar hybrid supported lipid bilayers Thiol-gold linkages Antibody covalent coupling Inhibition of nonspecific adsorption Immunosensors Surface plasmon resonance |
| ISSN号 | 0927-7765 |
| 产权排序 | [Almeida, I.; Marques, J. T.; de Almeida, R. F. M.; Viana, A. S.] Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, P-1749016 Lisbon, Portugal; [Liu, W.; Niu, Y.; Jin, G.] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Viana, AS (reprint author), Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, Edificio C8, P-1749016 Lisbon, Portugal. |
| 中文摘要 | In this work, a simple yet robust method to prepare lipid-based biosensing interfaces on gold using common lipids (a phospholipid and cholesterol) and an alkanethiol is reported. The lipids were carefully chosen to tailor the biophysical properties of the bilayer. The simplicity of the method relies on the incorporation of a small percentage of decanethiol in the lipid vesicles for a direct formation of a thiol-linked supported lipid bilayer, which is advantageous in several respects. It prevents the use of specially synthesized thiolipids and preserves the natural fluidity and dynamics of the lipids. As a consequence the whole arrangement is extremely stable regarding ionic strength changes and solution flow during surface plasmon resonance experiments. Moreover, we show that this interface is very effective on suppressing the nonspecific adsorption of proteins on the surface, and enables the covalent attachment of the recognition antibody. The subsequent detection of specific interaction toward antigen was monitored in real-time by SPR and confirmed by ellipsometric measurements. This lipid-based biosensing platform is versatile and can be adapted to the biorecognition reaction of interest. (C) 2015 Elsevier B.V. All rights reserved. |
| 类目[WOS] | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biophysics ; Chemistry ; Materials Science |
| 关键词[WOS] | SURFACE-PLASMON RESONANCE ; BILAYER-LIPID MEMBRANES ; PHOSPHOLIPID-BILAYERS ; PHASE-EQUILIBRIA ; MODIFIED GOLD ; CHOLESTEROL ; IMMOBILIZATION ; MONOLAYERS ; RAFTS ; PHOSPHATIDYLCHOLINE |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1016/j.colsurfb.2015.10.048 |
| 语种 | 英语 |
| WOS记录号 | WOS:000367408100121 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58654]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Almeida I,Marques JT,Liu W,et al. Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2015,136:997-1003. |
| APA | Almeida I.,Marques JT.,Liu W.,Niu Y.,de Almeida RFM.,...&Viana AS.(2015).Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces.COLLOIDS AND SURFACES B-BIOINTERFACES,136,997-1003. |
| MLA | Almeida I,et al."Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces".COLLOIDS AND SURFACES B-BIOINTERFACES 136(2015):997-1003. |
入库方式： OAI收割
来源：力学研究所
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