Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique
文献类型：期刊论文
| 作者 | Yang H; Tong CF ; Fu CL; Xu YH; Liu XF; Chen Q; Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Long M(龙勉)
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| 刊名 | JOURNAL OF IMMUNOLOGICAL METHODS
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| 出版日期 | 2016-01 |
| 卷号 | 428页码:20-29 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | Nucleated cells Movement Contact Adhesion Pipette |
| ISSN号 | 0022-1759 |
| 产权排序 | Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; Chinese Acad Sci, Beijing Key Lab Engn Construct & Mech, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China. |
| 中文摘要 | Adhesion between two nucleated cells undergoes specific significances in immune responses and tumor metastasis since cellular adhesive molecules usually express on two apposed cell membranes. However, quantification of the interactions between two nucleated cells is still challenging in microvasculature. Here distinct cell systems were used, including three types of human cells (Jurkat cell or PMN vs. MDA-MB-231 cell) and two kinds of murine native cells (PMN vs. liver sinusoidal endothelial cell). Cell movement, compression to, and relaxation from the counterpart cell were quantified using an in-house developed gas-driven micropipette aspiration technique (GDMAT). This assay is robust to quantify this process since cell movement and contact inside a pipette are independent of the repeated test cycles. Measured approaching or retraction velocity follows well a normal distribution, which is independent on the cycle period. Contact area or duration also fits a Gaussian distribution and moreover contact duration is linearly correlated with the cycle period. Cell movement is positively related to gas flux but negatively associated to medium viscosity. Cell adhesion tends to reach an equilibrium state with increase of cycle period or contact duration. These results further the understanding in the dynamics of cell movement and contact in microvasculature. (C) 2015 Elsevier B.V. All rights reserved. |
| 分类号 | Q3 |
| 类目[WOS] | Biochemical Research Methods ; Immunology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 关键词[WOS] | THERMAL BARRIER COATINGS ; FRACTURE CHARACTERISTICS ; MECHANICAL-PROPERTIES ; ACOUSTIC-EMISSION ; EVOLUTION ; THICKNESS ; TENSILE ; ENERGY ; TESTS |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.jim.2015.11.007 |
| 语种 | 英语 |
| WOS记录号 | WOS:000369451600003 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58683]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Yang H,Tong CF,Fu CL,et al. Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2016,428:20-29. |
| APA | Yang H.,Tong CF.,Fu CL.,Xu YH.,Liu XF.,...&龙勉.(2016).Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique.JOURNAL OF IMMUNOLOGICAL METHODS,428,20-29. |
| MLA | Yang H,et al."Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique".JOURNAL OF IMMUNOLOGICAL METHODS 428(2016):20-29. |
入库方式： OAI收割
来源：力学研究所
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