A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate
文献类型：期刊论文
| 作者 | Zhao, Kexin ; Guo, Xiaohong; Gong, Jun
|
| 刊名 | JOURNAL OF ENVIRONMENTAL BIOLOGY
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 34期号:2页码:401-407 |
| 关键词 | PAR degradation Rhodococcus Catechol 1 Cat A gene Cloning Transmition 2-dioxygenase |
| 英文摘要 | The gene encoding catechol 1, 2-dioxygenase (catA) is one of the common biomarkers used for evaluation of microbial degradation of aromatic compounds and bioremediation potentials, whereas the expression of the gene has been little studied. The diversity of the catA genes and their expression pattern in a novel Rhodococcus sp. strain LIN isolated from coastal sediments of the Bohai Sea, China, were investigated. We found that this strain exhibited catechol 1, 2-dioxygenase activity when cultivated with benzoate as the sole carbon source. The catA genes were amplified with degenerate primers and sequenced. Sequence analyses show that the strain has three catA genes which are highly similar to each other (> 97%). However, only one gene was transcribed according to reverse transcription-polymerase chain reaction (RT-PCR) analysis. This indicates that RNA-based approaches could better reflect the diversity and scale of the environmental microbial function than DNA-based ones, when sequencing and quantifying (e.g. quantitative PCR) of the catA gene are employed to assess the environmental pollutions and bioremediations. |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | MARINE ROSEOBACTER LINEAGE ; BETA-KETOADIPATE PATHWAY ; CATECHOL 1,2-DIOXYGENASE ; PHENOL ; ANILINE ; BIODEGRADATION ; DEGRADATION ; BACTERIUM ; IDENTIFICATION ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323852700017 |
| 公开日期 | 2016-04-26 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9052]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Lab Environm Microbiol,Network Lab Coastal Microb, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Kexin,Guo, Xiaohong,Gong, Jun. A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate[J]. JOURNAL OF ENVIRONMENTAL BIOLOGY,2013,34(2):401-407. |
| APA | Zhao, Kexin,Guo, Xiaohong,&Gong, Jun.(2013).A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate.JOURNAL OF ENVIRONMENTAL BIOLOGY,34(2),401-407. |
| MLA | Zhao, Kexin,et al."A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate".JOURNAL OF ENVIRONMENTAL BIOLOGY 34.2(2013):401-407. |
入库方式： OAI收割
来源：烟台海岸带研究所
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