Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb
文献类型：期刊论文
| 作者 | Zhou, Yuan1,2; Shao Hong-bo1,2,3,4,5; Chen, De-yu1,2 |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2009-10-19 |
| 卷号 | 8期号:20页码:5208-5214 |
| 关键词 | Grifola umbellate Chinese medicinal herb. polysaccharides submerged culture organic nitrogen source carbon source orthogonal design |
| 英文摘要 | Grifola umbellate is a famous and expensive Chinese herb medicine and the main medicinal component is polysaccharide mainly produced by its mycelia. Effects of organic nitrogen and carbon resources on mycelial growth and polysaccharides production of a medicinal mushroom, G. umbellate were studied in the submerged culture system. Soybean meal was selected as the optimal organic nitrogen source for its significant promotion effects on mycelial growth and polysaccharides synthesis based on results of an orthogonal matrix experiment design. Glucose was proved to be a preferred carbon source by comparison with fructose and sucrose. The optimal dosages of soybean meal 5.63% (w/v) and glucose 4.33% (w/v) were obtained, respectively through the curvilinear regressions experiment. The productions of mycelial dry weight, exo-polysaccharide (EPS) and inner polysaccharide (IPS), in the optimal medium, were about 3.16, 1.48 and 2.78 folds higher than those in the basal medium, respectively and culture duration was shortened to half through optimizing the organic nitrogen and carbon sources. These results will be significant contribution to process biochemistry of medicinal fungus in the submerged culture system. |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273276200012 |
| 公开日期 | 2016-04-26 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9226]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.NW A&F Univ, Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China 2.NW A&F Univ, Minist Water Resources, Yangling 712100, Peoples R China 3.Binzhou Univ, Shandong Prov Key Lab Ecoenvironm Sci Yellow Rive, Binzhou 256603, Peoples R China 4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China 5.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yuan,Shao Hong-bo,Chen, De-yu. Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(20):5208-5214. |
| APA | Zhou, Yuan,Shao Hong-bo,&Chen, De-yu.(2009).Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(20),5208-5214. |
| MLA | Zhou, Yuan,et al."Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.20(2009):5208-5214. |
入库方式： OAI收割
来源：烟台海岸带研究所
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