Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins
文献类型：期刊论文
| 作者 | Li, HJ; Liu, Y ; Jin, HZ; Liu, SJ; Fang, ST; Wang, CH; Xia, CH
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2015-12-15 |
| 卷号 | 1007页码:23-29 |
| ISSN号 | 1570-0232 |
| 关键词 | Macroporous resins Vitexin-4 ''-O-glucoside Vitexin-2 ''-O-rhamnoside Purification Hawthorn leaves |
| 通讯作者 | Xia, CH (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. chunhuawang508@126.com ; chxia@yic.ac.cn |
| 产权排序 | [Li, Hongjuan; Liu, Ying; Liu, Sujing; Fang, Shengtao; Xia, Chuanhai] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Li, Hongjuan; Wang, Chunhua] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Jin, Haizhu] Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China; [Li, Hongjuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 中文摘要 | Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved. |
| 英文摘要 | Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | UPLC-ESI-MS/MS ; PREPARATIVE SEPARATION ; CHLOROGENIC ACID ; FLAVONOIDS ; PURIFICATION ; ENRICHMENT ; HPLC ; VITEXIN-2-O-RHAMNOSIDE ; ADSORPTION ; VITEXIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366772600004 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9308]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, HJ,Liu, Y,Jin, HZ,et al. Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,1007:23-29. |
| APA | Li, HJ.,Liu, Y.,Jin, HZ.,Liu, SJ.,Fang, ST.,...&Xia, CH.(2015).Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1007,23-29. |
| MLA | Li, HJ,et al."Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1007(2015):23-29. |
入库方式： OAI收割
来源：烟台海岸带研究所
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