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中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress

文献类型:期刊论文

作者Wang, HY; Tang, XL; Wang, HL; Shao, HB
刊名FRONTIERS IN PLANT SCIENCE
出版日期2015-09-29
卷号6
关键词Kosteletzlcya virginica salt stress proline metabolism proline accumulation expression profiles
ISSN号1664-462X
产权排序[Wang, Hongyan; Tang, Xiaoli; Shao, Hong-Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Wang, Hongyan; Wang, Honglei] China Agr Univ, Yantai Acad, Yantai, Peoples R China; [Wang, Hongyan; Tang, Xiaoli] Univ Chinese Acad Sci, Beijing, Peoples R China; [Shao, Hong-Bo] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing, Jiangsu, Peoples R China
通讯作者Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. shaohongbochu@126.com
中文摘要Proline accumulation is a common response to salt stress in many plants. Salt stress also increased proline concentration in roots, stems, and leaves of Kosteletzkya virginica seedling treated with 300 mM NaCl for 24 h and reached 3.75-, 4.76-, and 6.83- fold higher than controls. Further study on proline content in leaves under salt stress showed that proline content increased with increasing NaCl concentrations or time. The proline level peaked at 300 mM NaCl for 24 h and reached more than sixfold higher than control, but at 400 mM NaCl for 24 h proline content fell back slightly along with wilting symptom. To explore the cause behind proline accumulation, we first cloned full length genes related to proline metabolism including KvP5CS1, KvOAT, KvPDH, and KvProT from K. virginica and investigated their expression profiles. The results revealed that the expressions of KvP5CS1 and KvProT were sharply up-regulated by salt stress and the expression of KvOAT showed a slight increase with increasing salt concentrations or time, while the expression of KvPDH was not changed much and slightly decreased before 12 h and then returned to the original level. As the key enzyme genes for proline biosynthesis, the up-regulated expression of KvP5CS1 played a more important role than KvOAT for proline accumulation in leaves under salt stress. The low expression of KvPDH for proline catabolism also made a contribution to proline accumulation before 12 h.
英文摘要Proline accumulation is a common response to salt stress in many plants. Salt stress also increased proline concentration in roots, stems, and leaves of Kosteletzkya virginica seedling treated with 300 mM NaCl for 24 h and reached 3.75-, 4.76-, and 6.83- fold higher than controls. Further study on proline content in leaves under salt stress showed that proline content increased with increasing NaCl concentrations or time. The proline level peaked at 300 mM NaCl for 24 h and reached more than sixfold higher than control, but at 400 mM NaCl for 24 h proline content fell back slightly along with wilting symptom. To explore the cause behind proline accumulation, we first cloned full length genes related to proline metabolism including KvP5CS1, KvOAT, KvPDH, and KvProT from K. virginica and investigated their expression profiles. The results revealed that the expressions of KvP5CS1 and KvProT were sharply up-regulated by salt stress and the expression of KvOAT showed a slight increase with increasing salt concentrations or time, while the expression of KvPDH was not changed much and slightly decreased before 12 h and then returned to the original level. As the key enzyme genes for proline biosynthesis, the up-regulated expression of KvP5CS1 played a more important role than KvOAT for proline accumulation in leaves under salt stress. The low expression of KvPDH for proline catabolism also made a contribution to proline accumulation before 12 h.
研究领域[WOS]Plant Sciences
关键词[WOS]HIGH-YIELDING GENOTYPES ; MORUS-ALBA L. ; 1ST 2 STEPS ; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE ; ARABIDOPSIS-THALIANA ; TRANSCRIPTIONAL REGULATION ; DEHYDROGENASE GENES ; PLANT DEVELOPMENT ; GLYCINE BETAINE ; OSMOTIC-STRESS
收录类别SCI
语种英语
WOS记录号WOS:000362372400001
源URL[http://ir.yic.ac.cn/handle/133337/9754]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式
GB/T 7714
Wang, HY,Tang, XL,Wang, HL,et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. FRONTIERS IN PLANT SCIENCE,2015,6.
APA Wang, HY,Tang, XL,Wang, HL,&Shao, HB.(2015).Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress.FRONTIERS IN PLANT SCIENCE,6.
MLA Wang, HY,et al."Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress".FRONTIERS IN PLANT SCIENCE 6(2015).

入库方式: OAI收割

来源:烟台海岸带研究所

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