A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo
文献类型：期刊论文
| 作者 | Liu, P; Jing, XT; Yu, FB; Lv, CJ; Chen, LX
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| 刊名 | ANALYST
 |
| 出版日期 | 2015 |
| 卷号 | 140期号:13页码:4576-4583 |
| 产权排序 | [Liu, Ping; Jing, Xiaotong; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Fabiao; Lv, Changjun; Chen, Lingxin] Binzhou Med Univ, Med Res Ctr, Yantai 264003, Peoples R China |
| 通讯作者 | Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. fbyu@yic.ac.cn ; lxchen@yic.ac.cn |
| 英文摘要 | Nitroxyl (HNO), the one-electron reduced and protonated analogue of nitric oxide (NO), demonstrates distinctive bio-pharmacological effects in the treatment of cardiovascular disorders. Herein, we design and synthesize a near-infrared (NIR) metal-free fluorescent probe Cyto-JN for the detection of nitroxyl (HNO) in living cells and in vivo. The metal-free Cyto-JN is composed of two moieties: the Aza-BODIPY fluorophore and the HNO recognition unit, the diphenylphosphinobenzoyl group. Cyto-JN can react with HNO in a 1 : 1 stoichiometry, which may bring great benefit to the detection efficiency of bioassays. Cyto-JN shows high sensitivity toward HNO and exhibits low cytotoxic effect on cells. Moreover, the probe displays good selectivity for the detection of HNO in the presence of various biologically related species. Cyto-JN can be applied successfully to bio-imaging of HNO in living cells and in mice. The results of flow cytometry confirm that the probe Cyto-JN can be used to detect intracellular HNO qualitatively and quantitatively. |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NITRIC-OXIDE ; NITROXYL HNO ; AQUEOUS-SOLUTION ; HYDROGEN POLYSULFIDES ; REDUCTANT-RESISTANT ; ANGELIS SALT ; NO ; PEROXYNITRITE ; QUANTITATION ; CHEMISTRY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356171400030 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9258]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, P,Jing, XT,Yu, FB,et al. A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo[J]. ANALYST,2015,140(13):4576-4583. |
| APA | Liu, P,Jing, XT,Yu, FB,Lv, CJ,&Chen, LX.(2015).A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo.ANALYST,140(13),4576-4583. |
| MLA | Liu, P,et al."A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo".ANALYST 140.13(2015):4576-4583. |
入库方式： OAI收割
来源：烟台海岸带研究所
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