Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity
文献类型：期刊论文
| 作者 | Wang, SS; Chen, ZP ; Choo, J; Chen, LX
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| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 408期号:4页码:1015-1022 |
| 关键词 | Gold nanoparticles Peroxidase-like activity Colorimetric immunoassay Human IgG Gold enhancement |
| ISSN号 | 1618-2642 |
| 产权排序 | [Wang, Shasha; Chen, Zhaopeng; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Choo, Jaebum] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea; [Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 英文摘要 | A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3',5,5'-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H2O2. Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7-100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3 sigma/S for UV-vis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | LINKED-IMMUNOSORBENT-ASSAY ; COLORIMETRIC IMMUNOASSAY ; HORSERADISH-PEROXIDASE ; IMMUNOGLOBULIN-G ; NANOPARTICLES ; IGG ; REDUCTION ; GRAPHENE ; LABELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000368720600001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9292]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 2.Hanyang Univ, Dept Bionano Technol 3.Univ Chinese Acad Sci |
| 推荐引用方式 GB/T 7714 | Wang, SS,Chen, ZP,Choo, J,et al. Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(4):1015-1022. |
| APA | Wang, SS,Chen, ZP,Choo, J,&Chen, LX.(2016).Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(4),1015-1022. |
| MLA | Wang, SS,et al."Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.4(2016):1015-1022. |
入库方式： OAI收割
来源：烟台海岸带研究所
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