Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase
文献类型：期刊论文
| 作者 | Zhang, ZY ; Chen, ZP; Wang, SS; Cheng, FB; Chen, LX
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2015-12-23 |
| 卷号 | 7期号:50页码:27639-27645 |
| 关键词 | gold nanorods etching iodine alkaline phosphatase plasmonic ELISA |
| ISSN号 | 1944-8244 |
| 产权排序 | [Zhang, Zhiyang; Chen, Zhaopeng; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Zhang, Zhiyang; Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cheng, Fangbin] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China |
| 通讯作者 | Chen, ZP (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. zhpchen@yic.ac.cn |
| 英文摘要 | Here, we propose a plasmonic enzyme-linked immunosorbent assay (ELISA) based on highly sensitive colorimetric detection of alkaline phosphatase (ALP), which is achieved by iodine-mediated etching of gold nanorods (AuNRs). Once the sandwich-type immunocomplex is formed, the ALP bound on the polystyrene microwells will hydrolyze ascorbic acid 2-phosphate into ascorbic acid. Subsequently, iodate is reduced to iodine, a moderate oxidant, which etches AuNRs from rod to sphere in shape. The shape change of AuNRs leads to a blue-shift of longitudinal localized surface plasmon resonance. As a result, the solution of AuNRs changes from blue to red. Benefiting from the highly sensitive detection of ALP, the proposed plasmonic ELISA has achieved an ultralow detection limit (100 pg/mL) for human immunoglobulin G (IgG). Importantly, the visual detection limit (3.0 ng/mL) allows the rapid differential diagnosis with the naked eye. The further detection of human IgG in fetal bovine serum indicates its applicability to the determination of low abundance protein in complex biological samples. |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| 关键词[WOS] | VISUAL DETECTION ; ULTRASENSITIVE DETECTION ; SILVER ENHANCEMENT ; NANOPARTICLES ; IMMUNOASSAY ; ASSAY ; AGGREGATION ; GLUCOSE ; AMPLIFICATION ; SPECTROSCOPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367561500010 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9299]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, ZY,Chen, ZP,Wang, SS,et al. Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(50):27639-27645. |
| APA | Zhang, ZY,Chen, ZP,Wang, SS,Cheng, FB,&Chen, LX.(2015).Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase.ACS APPLIED MATERIALS & INTERFACES,7(50),27639-27645. |
| MLA | Zhang, ZY,et al."Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase".ACS APPLIED MATERIALS & INTERFACES 7.50(2015):27639-27645. |
入库方式： OAI收割
来源：烟台海岸带研究所
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