中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin

文献类型:期刊论文

作者Wang, XY; Yu, JL; Kang, Q; Shen, DZ; Li, JH; Chen, LX
刊名BIOSENSORS & BIOELECTRONICS
出版日期2016-03-15
卷号77页码:624-630
关键词Molecular imprinting Ratiometric fluorescence Fluorescence resonance energy transfer Phycocyanin
ISSN号0956-5663
产权排序[Wang, Xiaoyan; Yu, Jialuo; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China; [Wang, Xiaoyan; Yu, Jialuo; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
通讯作者Shen, DZ (reprint author), Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China. dzshen@sdnu.edu.cn ; lxchen@yic.ac.cn
英文摘要A facile strategy was developed to prepare molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin (PC) based on fluorescence resonance energy transfer (FRET), via a sal-gel polymerization process using nitrobenzoxadiazole (NBD) as fluorescent signal source. The ratio of two fluorescence peak emission intensities of NBD and PC was utilized to determine the concentration of PC, which could effectively reduce the background interference and fluctuation of diverse conditions. As a result, this sensor obtained high sensitivity with a low detection limit of 0.14 nM within 6 min, and excellent recognition specificity for PC over its analogues with a high imprinting factor of 9.1. Furthermore, the sensor attained high recoveries in the range of 93.8-110.2% at three spiking levels of PC, with precisions below 4.7% in seawater and lake water samples. The developed sensor strategy demonstrated simplicity, reliability, rapidity, high selectivity and high sensitivity, proving to be a feasible way to develop high efficient fluorescence sensors and thus potentially applicable for ultratrace analysis of complicated matrices. (C) 2015 Elsevier B.V. All rights reserved.
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]QUANTUM DOTS ; IONS ; NANOPARTICLES ; POLYMER ; MONOMER ; HYBRID ; PROBE ; SITE ; TNT
收录类别SCI
语种英语
WOS记录号WOS:000366766900090
源URL[http://ir.yic.ac.cn/handle/133337/9314]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat
3.Binzhou Med Univ, Sch Pharm
推荐引用方式
GB/T 7714
Wang, XY,Yu, JL,Kang, Q,et al. Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin[J]. BIOSENSORS & BIOELECTRONICS,2016,77:624-630.
APA Wang, XY,Yu, JL,Kang, Q,Shen, DZ,Li, JH,&Chen, LX.(2016).Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin.BIOSENSORS & BIOELECTRONICS,77,624-630.
MLA Wang, XY,et al."Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin".BIOSENSORS & BIOELECTRONICS 77(2016):624-630.

入库方式: OAI收割

来源:烟台海岸带研究所

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