Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum
文献类型：期刊论文
| 作者 | Wang, Q ; Zhang, LB; Yang, DL; Yu, Q; Li, F; Cong, M; Ji, CL; Wu, HF; Zhao, JM
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2015-11-01 |
| 卷号 | 47期号:1页码:302-312 |
| 关键词 | Defensin Manila clam Diversity Positive selection |
| ISSN号 | 1050-4648 |
| 产权排序 | [Wang, Qing; Yang, Dinglong; Yu, Qian; Li, Fei; Cong, Ming; Ji, Chenglong; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Environm Processes & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, Linbao] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong; Yu, Qian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Processes & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn |
| 英文摘要 | Four types of defensins were identified in Manila clam and designated as Rpdef1, Rpdef2, Rpdef3 and Rpdef4, which encoded a polypeptide of 49, 46, 45 and 42 amino acids, respectively. Sequence alignments indicated that Rpdef1 shared 46.9% identity with Rpdef2, 40.8% with Rpdef3, and 34.7% with Rpdef4. Analysis of transcript polymorphism showed that Rpdef3 accounted for about 60% frequency of Rpdefs occurrence in clams from three geographic origins (Dalian, Qingdao and Hangzhou). By quantitative real-time RT-PCR (qRT-PCR) analysis, the transcripts of Rpdefs were mainly detected in hemocytes and they responded sensitively to bacterial challenge in hemocytes. Evolutionary analysis indicated that all Rpdefs were under positive selection with positively selected basic amino acid residues detected in the C-terminal regions, which perhaps have a functional relevance by modifying the charge distribution of Rpdefs. The results also showed some lineages with dN/dS > 1, suggesting positive selection pressures existed in some lineages of phylogeny tree constructed by mollusk defensins. Overall, our results suggest that Rpdefs perhaps played important roles in host defense and positive selection is the major driving force in generating high diversity of defensins in the Manila clam. (C) 2015 Elsevier Ltd. All rights reserved. |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | MUSSEL MYTILUS-GALLOPROVINCIALIS ; OYSTER CRASSOSTREA-GIGAS ; ANTIMICROBIAL PEPTIDES ; MEDITERRANEAN MUSSEL ; POSITIVE SELECTION ; RAPID EVOLUTION ; INNATE IMMUNITY ; PEARL MUSSEL ; MYTICIN-C ; HEMOCYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365053300035 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/9539]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_污染过程与控制实验室 海岸带生物资源高效利用研究与发展中心 |
| 推荐引用方式 GB/T 7714 | Wang, Q,Zhang, LB,Yang, DL,et al. Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):302-312. |
| APA | Wang, Q.,Zhang, LB.,Yang, DL.,Yu, Q.,Li, F.,...&Zhao, JM.(2015).Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,47(1),302-312. |
| MLA | Wang, Q,et al."Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):302-312. |
入库方式： OAI收割
来源：烟台海岸带研究所
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