Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat
文献类型：期刊论文
| 作者 | Liu, Caixiang1; Ding, Feng4; Hao, Fuhua1; Yu, Men1,5; Lei, Hehua1; Wu, Xiangyu1; Zhao, Zhengxi6; Guo, Hongxiang7; Yin, Jun7; Wang, Yulan1,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-02-10 |
| 卷号 | 6 |
| 英文摘要 | Pre-harvest sprouting (PHS) is a worldwide problem for wheat production and transgene antisense-thioredoxin-s (anti-trx-s) facilitates outstanding resistance. To understand the molecular details of PHS resistance, we analyzed the metabonomes of the transgenic and wild-type (control) wheat seeds at various stages using NMR and GC-FID/MS. 60 metabolites were dominant in these seeds including sugars, organic acids, amino acids, choline metabolites and fatty acids. At day-20 post-anthesis, only malate level in transgenic wheat differed significantly from that in controls whereas at day-30 post-anthesis, levels of amino acids and sucrose were significantly different between these two groups. For mature seeds, most metabolites in glycolysis, TCA cycle, choline metabolism, biosynthesis of proteins, nucleotides and fatty acids had significantly lower levels in transgenic seeds than in controls. After 30-days post-harvest ripening, most metabolites in transgenic seeds had higher levels than in controls including amino acids, sugars, organic acids, fatty acids, choline metabolites and NAD+. These indicated that anti-trx-s lowered overall metabolic activities of mature seeds eliminating pre-harvest sprouting potential. Post-harvest ripening reactivated the metabolic activities of transgenic seeds to restore their germination vigor. These findings provided essential molecular phenomic information for PHS resistance of anti-trx-s and a credible strategy for future developing PHS resistant crops. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | METABONOMIC ANALYSIS ; PHOSPHOLIPASE-D ; BARLEY-GRAIN ; THIOREDOXIN ; NMR ; PROTEIN ; EXPRESSION ; RESPONSES ; IDENTIFICATION ; DEHYDROGENASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369741600001 |
| 公开日期 | 2016-04-26 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9189]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Fudan Univ, Collaborat Innovat Ctr Genet & Dev Biol, State Key Lab Genet Engn, Metabon & Syst Biol Lab,Sch Life Sci, Shanghai 200438, Peoples R China 3.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 5.Wuhan Zhongke Metaboss Ltd, Wuhan 430074, Peoples R China 6.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 7.Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Caixiang,Ding, Feng,Hao, Fuhua,et al. Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Liu, Caixiang.,Ding, Feng.,Hao, Fuhua.,Yu, Men.,Lei, Hehua.,...&Tang, Huiru.(2016).Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat.SCIENTIFIC REPORTS,6. |
| MLA | Liu, Caixiang,et al."Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：武汉物理与数学研究所
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