Antioxidant Drug Ternpol Promotes Functional Metabolic Changes in the Gut Microbiota
文献类型：期刊论文
| 作者 | Cai, Jingwei1; Zhang, Limin1,2; Jones, Richard A.1; Correll, Jared B.1; Hatzakis, Emmanuel4; Smith, Philip B.3; Gonzalez, Frank J.5; Patterson, Andrew D.1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 15期号:2页码:563-571 |
| 关键词 | SCFAs metabolomics GC-MS NMR bomb calorimetry tempol obesity |
| 英文摘要 | Recent studies have identified the important role of the gut microbiota in the pathogenesis and progression of obesity and related metabolic disorders. The antioxidant tempol was shown to prevent or reduce weight gain and modulate the gut microbiota community in mice; however, the mechanism by which tempol modulates weight gain/loss with respect to the host and gut microbiota has not been clearly established. Here we show that tempol (0, 1, 10, and 50 mg/kg p.o. for 5 days) decreased cecal bacterial fermentation and increased fecal energy excretion in a dose-dependent manner. Liver H-1 NMR-based metabolomics identified a dose dependent decrease in glycogen and glucose, enhanced glucogenic and ketogenic activity (tyrosine and phenylalanine), and increased activation of the glycolysis pathway. Serum H-1 NMR-based metabolomics indicated that tempol promotes enhanced glucose catabolism. Hepatic gene expression was significantly altered as demonstrated by an increase in Pepck and G6pase and a decrease in Hnf4a, ChREBP, Fabp1, and Cd36 mRNAs. No significant change in the liver and serum metabolomic profiles was observed in germ-free mice, thus establishing a significant role for the gut microbiota in mediating the beneficial metabolic effects of tempol. These results demonstrate that tempol modulates the gut microbial community and its function, resulting in reduced host energy availability and a significant shift in liver metabolism toward a more catabolic state. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CHAIN FATTY-ACIDS ; OBESITY ; ACETATE ; GLUCOSE ; WEIGHT ; RATS ; ECOLOGY ; IMPACTS ; DISEASE ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369771700020 |
| 公开日期 | 2016-04-26 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9191]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Penn State Univ, Ctr Mol Toxicol & Carcinogenesis, Dept Vet & Biomed Sci, State Coll, PA 16802 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, CAS Key Lab Magnet Resonance Biol Syst,State Key, Wuhan 430071, Peoples R China 3.Penn State Univ, Metabol Facil, Huck Inst Life Sci, State Coll, PA 16802 USA 4.Penn State Univ, Dept Chem, State Coll, PA 16802 USA 5.NCI, Lab Metab, NIH, Bethesda, MD 20892 USA |
| 推荐引用方式 GB/T 7714 | Cai, Jingwei,Zhang, Limin,Jones, Richard A.,et al. Antioxidant Drug Ternpol Promotes Functional Metabolic Changes in the Gut Microbiota[J]. JOURNAL OF PROTEOME RESEARCH,2016,15(2):563-571. |
| APA | Cai, Jingwei.,Zhang, Limin.,Jones, Richard A..,Correll, Jared B..,Hatzakis, Emmanuel.,...&Patterson, Andrew D..(2016).Antioxidant Drug Ternpol Promotes Functional Metabolic Changes in the Gut Microbiota.JOURNAL OF PROTEOME RESEARCH,15(2),563-571. |
| MLA | Cai, Jingwei,et al."Antioxidant Drug Ternpol Promotes Functional Metabolic Changes in the Gut Microbiota".JOURNAL OF PROTEOME RESEARCH 15.2(2016):563-571. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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