Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens
文献类型：期刊论文
| 作者 | Zhang,Zhijun; Guan,Yijia; Li,Meng; Zhao,Andong; Ren,Jinsong; Qu,Xiaogang |
| 刊名 | chemical science
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:5页码:2822-2826 |
| 关键词 | CERIUM OXIDE NANOPARTICLES ULTRASENSITIVE DETECTION BORONATE AFFINITY LABEL-FREE ENZYME-INHIBITORS THIN-FILMS NAKED EYE RECOGNITION POLYMERS CELLS |
| 通讯作者 | qu,xg |
| 英文摘要 | sandwich elisa methods have been widely used for biomarker and pathogen detection because of their high specificity and sensitivity. however, the main drawbacks of this assay are the cost, the time-consuming procedure for the isolation of antibodies and their poor stability. to overcome these restrictions, we herein fabricated artificial antibodies based on imprinting technology and developed a sandwich elisa for pathogen detection. both the capture and detection antibodies were obtained via an in situ method, with simplicity, rapidity and low cost. the peroxidase mimics, the ceo2 nanoparticles, as signal generators were integrated with the detection antibody. the fabricated artificial antibodies exhibited not only natural antibody-like binding affinities and selectivities, but also superior stability and reusability. the detection limit was about 500 cfu ml(-1), which is much lower than that of traditional elisa methods (10(4) to 10(5) cfu ml(-1)). furthermore, the capture antibody can disinfect pathogens in situ. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64577]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang,Zhijun,Guan,Yijia,Li,Meng,et al. Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens[J]. chemical science,2015,6(5):2822-2826. |
| APA | Zhang,Zhijun,Guan,Yijia,Li,Meng,Zhao,Andong,Ren,Jinsong,&Qu,Xiaogang.(2015).Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens.chemical science,6(5),2822-2826. |
| MLA | Zhang,Zhijun,et al."Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens".chemical science 6.5(2015):2822-2826. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。