Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering
文献类型：期刊论文
| 作者 | Ren,Kaixuan; He,Chaoliang; Xiao,Chunsheng; Li,Gao; Chen,XS |
| 刊名 | biomaterials
 |
| 出版日期 | 2015 |
| 卷号 | 51期号:7页码:238-249 |
| 关键词 | MESENCHYMAL STEM-CELLS BIOMEDICAL APPLICATIONS REGENERATIVE MEDICINE CLICK GLYCOSYLATION MICHAEL ADDITION DRUG-DELIVERY SITU POLYPEPTIDES REPAIR BONE |
| 通讯作者 | he,cl |
| 英文摘要 | glycopolypeptides are an emerging class of bioinspired polymers that mimic naturally occurring glycopeptides or glycoproteins, and therefore are expected to exhibit great potential for biomedical applications. in this study, a glycopolypeptide was synthesized by conjugation of poly(gamma-propargyl-l-glutamate) (pplg) with azido-modified mannose and 3-(4-hydroxyphenyl) propanamide (hppa), via click chemistry. injectable hydrogels based on the glycopolypeptide were developed through enzymatic crosslinking reaction in the presence of horseradish peroxidase (hrp) and hydrogen peroxide (h2o2). the physicochemical properties of the hydrogels, such as gelation time, storage modulus, swelling and degradation time, could be controlled by varying the concentrations of hrp and h2o2. the glycopolypetide copolymer as well as the extracts of the glycopolypetide hydrogels displayed good cytocompatibility in vitro. after subcutaneous injection into rats, the glycopolypeptide hydrogels were rapidly formed in situ, and exhibited acceptable biocompatibility accompanying the degradation of the hydrogels in vivo. the rabbit chondrocytes inside the glycopolypeptide hydrogels showed spherical morphology with high viability during the incubation period of 3 weeks in vitro, and exhibited a higher proliferation rate than within the hydrogel counterparts of pplg grafted with 2-(2-(2-methoxyethoxy)ethoxy)ethane (meo3) and hppa. biochemical analysis demonstrated that the production of glycosaminoglycans (gag) and type ii collagen were significantly enhanced after incubation for 2 and 3 weeks in vitro. moreover, the chondrocyte-containing glycopolypeptide hydrogels in subcutaneous model of nude mice maintained chondrocyte phenotype and produced the cartilaginous specific matrix. these results indicated that the biomimetic glycopolypeptide-based hydrogels hold potential as three-dimensional scaffolds for cartilage tissue engineering. (c) 2015 elsevier ltd. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351796700023 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64587]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ren,Kaixuan,He,Chaoliang,Xiao,Chunsheng,et al. Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering[J]. biomaterials,2015,51(7):238-249. |
| APA | Ren,Kaixuan,He,Chaoliang,Xiao,Chunsheng,Li,Gao,&Chen,XS.(2015).Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering.biomaterials,51(7),238-249. |
| MLA | Ren,Kaixuan,et al."Injectable glycopolypeptide hydrogels as biomimetic scaffolds for cartilage tissue engineering".biomaterials 51.7(2015):238-249. |
入库方式： OAI收割
来源：长春应用化学研究所
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