Revealing the carbohydrate pattern on a cell surface by super-resolution imaging
文献类型：期刊论文
| 作者 | Chen,Junling; Gao,Jing; Wu,Jiazhen; Zhang,Min; Cai,Mingjun; Xu,Haijiao; Jiang,Junguang; Tian,Zhiyuan; Wang,Hongda |
| 刊名 | nanoscale
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:8页码:3373-3380 |
| 关键词 | OPTICAL RECONSTRUCTION MICROSCOPY FLUORESCENT-PROBES MEMBRANE GLYCANS LOCALIZATION RECOGNITION GLYCOMICS DISEASE STORM LIMIT |
| 通讯作者 | wang,hd |
| 英文摘要 | carbohydrates are involved in various physiological and pathological activities including cell adhesion, signal transduction and tumor invasion. the distribution of carbohydrates is the molecular basis of their multiple functions, but remains poorly understood. here, we employed direct stochastic optical reconstruction microscopy (dstorm) to visualize the pattern of n-acetylglucosamine (n-glcnac) on vero cell membranes at the nanometer level of resolution. we found that n-glcnacs exist in irregular clusters on the apical membrane with an average cluster area of about 0.37 mu m(2). most of these n-glcnac clusters are co-localized with lipid rafts by dual-color dstorm imaging, suggesting that carbohydrates are closely associated with lipid rafts as the functional domains. our results demonstrate that super-resolution imaging is capable of characterizing the distribution of carbohydrates on the cellular surface at the molecular level. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/64662]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen,Junling,Gao,Jing,Wu,Jiazhen,et al. Revealing the carbohydrate pattern on a cell surface by super-resolution imaging[J]. nanoscale,2015,7(8):3373-3380. |
| APA | Chen,Junling.,Gao,Jing.,Wu,Jiazhen.,Zhang,Min.,Cai,Mingjun.,...&Wang,Hongda.(2015).Revealing the carbohydrate pattern on a cell surface by super-resolution imaging.nanoscale,7(8),3373-3380. |
| MLA | Chen,Junling,et al."Revealing the carbohydrate pattern on a cell surface by super-resolution imaging".nanoscale 7.8(2015):3373-3380. |
入库方式： OAI收割
来源：长春应用化学研究所
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